Transcriptional repression by MYB3R proteins regulates plant organ growth

Kobayashi K, Suzuki T, Iwata E, Nakamichi N, Suzuki T, Chen P, Ohtani M, Ishida T, Hosoya H, Müller S, Leviczky T, Pettko-Szandtner A, Darula Z, Iwamoto A, Nomoto M, Tada Y, Higashiyama T, Demura T, Doonan JH, Hauser MT, Sugimoto K, Umeda M, Magyar Z, Boegre L, Ito M (2015)

Publication Type: Journal article

Publication year: 2015

Journal

EMBO Journal EMBO Press

Book Volume: 34

Pages Range: 1992-2007

Journal Issue: 15

DOI: 10.15252/embj.201490899

Abstract

In multicellular organisms, temporal and spatial regulation of cell proliferation is central for generating organs with defined sizes and morphologies. For establishing and maintaining the post-mitotic quiescent state during cell differentiation, it is important to repress genes with mitotic functions. We found that three of the Arabidopsis MYB3R transcription factors synergistically maintain G2/M-specific genes repressed in post-mitotic cells and restrict the time window of mitotic gene expression in proliferating cells. The combined mutants of the three repressor-type MYB3R genes displayed long roots, enlarged leaves, embryos, and seeds. Genome-wide chromatin immunoprecipitation revealed that MYB3R3 binds to the promoters of G2/M-specific genes and to E2F target genes. MYB3R3 associates with the repressor-type E2F, E2FC, and the RETINOBLASTOMA RELATED proteins. In contrast, the activator MYB3R4 was in complex with E2FB in proliferating cells. With mass spectrometry and pairwise interaction assays, we identified some of the other conserved components of the multiprotein complexes, known as DREAM/dREAM in human and flies. In plants, these repressor complexes are important for periodic expression during cell cycle and to establish a post-mitotic quiescent state determining organ size.

Authors with CRIS profile

Sabine Müller

Involved external institutions

Nagoya University / 名古屋大学 JP Japan (JP) Nara Institute of Science and Technology (NAIST) / 奈良先端科学技術大学院大学 JP Japan (JP) Kumamoto University / 熊本大学 JP Japan (JP) Tokyo Gakugei University JP Japan (JP) Eberhard Karls Universität Tübingen DE Germany (DE) Hungarian Academy of Sciences / Magyar Tudományos Akadémia (MTA) HU Hungary (HU) Biological Research Centre (BRC) of the Hungarian Academy of Sciences / MTA Szegedi Biológiai Központ HU Hungary (HU) Aberystwyth University GB United Kingdom (GB) Universität für Bodenkultur Wien (BOKU) / University of Natural Resources and Life Sciences, Vienna AT Austria (AT) Riken Institute of Physical and Chemical Research / 理化学研究所 JP Japan (JP) University College London (UCL) GB United Kingdom (GB)

How to cite

APA:

Kobayashi, K., Suzuki, T., Iwata, E., Nakamichi, N., Suzuki, T., Chen, P.,... Ito, M. (2015). Transcriptional repression by MYB3R proteins regulates plant organ growth. EMBO Journal, 34(15), 1992-2007. https://doi.org/10.15252/embj.201490899

MLA:

Kobayashi, Kosuke, et al. "Transcriptional repression by MYB3R proteins regulates plant organ growth." EMBO Journal 34.15 (2015): 1992-2007.

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