Analysis of phragmoplast kinetics during plant cytokinesis

Livanos P, Chugh M, Müller S (2017)

Publication Type: Book chapter / Article in edited volumes

Publication year: 2017


Publisher: Humana Press Inc.

Edited Volumes: Plant Protein Secretion

Series: Methods in Molecular Biology

City/Town: New York

Book Volume: 1662

Pages Range: 137-150

ISBN: 978-1-4939-7262-3

DOI: 10.1007/978-1-4939-7262-3_12


In plants, the partitioning of daughter cells during cytokinesis is achieved via physical insertion of a membranous cell plate within the dividing parent cell. It is a cellular process of extensive protein secretion and membrane trafficking toward the plane of cell division and the cytoskeleton is an important facilitator of this process. A specialized cytoskeletal array termed phragmoplast expands centrifugally throughout cytokinesis and directs, mostly Golgi-derived vesicles that ultimately fuse to form the developing cell plate. The function of the phragmoplast in guiding cell plate synthesis has strongly motivated many scientists to monitor its dynamic behavior. In this chapter, we present an overview of basic principles and methods concerning the live imaging of cytokinetic plant cells using confocal laser scanning microscopy (CLSM) and the analysis of phragmoplast expansion.

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Livanos, P., Chugh, M., & Müller, S. (2017). Analysis of phragmoplast kinetics during plant cytokinesis. In Liwen Jiang (Eds.), Plant Protein Secretion. (pp. 137-150). New York: Humana Press Inc..


Livanos, Pantelis, Mayank Chugh, and Sabine Müller. "Analysis of phragmoplast kinetics during plant cytokinesis." Plant Protein Secretion. Ed. Liwen Jiang, New York: Humana Press Inc., 2017. 137-150.

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