Designing of spider silk proteins for human induced pluripotent stem cell-based cardiac tissue engineering

Esser T, Trossmann VT, Lentz S, Engel F, Scheibel T (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Materials Today Bio Elsevier

Book Volume: 11

Article Number: 100114

DOI: 10.1016/j.mtbio.2021.100114

Abstract

Materials made of recombinant spider silk proteins are promising candidates for cardiac tissue engineering, and their suitability has so far been investigated utilizing primary rat cardiomyocytes. Herein, we expanded the tool box of available spider silk variants and demonstrated for the first time that human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes attach, contract, and respond to pharmacological treatment using phenylephrine and verapamil on explicit spider silk films. The hiPSC-cardiomyocytes contracted for at least 14 days on films made of positively charged engineered Araneus diadematus fibroin 4 (eADF4(κ16)) and three different arginyl-glycyl-aspartic acid (RGD)-tagged spider silk variants (positively or negatively charged and uncharged). Notably, hiPSC-cardiomyocytes exhibited different morphologies depending on the spider silk variant used, with less spreading and being smaller on films made of eADF4(κ16) than on RGD-tagged spider silk films. These results indicate that spider silk engineering is a powerful tool to provide new materials suitable for hiPSC-based cardiac tissue engineering.

Authors with CRIS profile

Tilman Esser Department of Nephropathology Felix Engel Professur für Experimentelle Nieren- und Kreislaufforschung

Involved external institutions

Universität Bayreuth DE Germany (DE)

How to cite

APA:

Esser, T., Trossmann, V.T., Lentz, S., Engel, F., & Scheibel, T. (2021). Designing of spider silk proteins for human induced pluripotent stem cell-based cardiac tissue engineering. Materials Today Bio, 11. https://doi.org/10.1016/j.mtbio.2021.100114

MLA:

Esser, Tilman, et al. "Designing of spider silk proteins for human induced pluripotent stem cell-based cardiac tissue engineering." Materials Today Bio 11 (2021).

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