A “Cell-Friendly” Window for the Interaction of Cells with Hyaluronic Acid/Poly-l-Lysine Multilayers
Madaboosi N, Uhlig K, Schmidt S, Vikulina AS, Moehwald H, Duschl C, Volodkin D (2018)
Publication Type: Journal article
Publication year: 2018
Journal
Book Volume: 18
Article Number: 1700319
Journal Issue: 2
Abstract
Polyelectrolyte multilayers assembled from hyaluronic acid (HA) and poly-l-lysine (PLL) are most widely studied showing excellent reservoir characteristics to host molecules of diverse nature; however, thick (HA/PLL)n films are often found cell repellent. By a systematic study of the adhesion and proliferation of various cells as a function of bilayer number “n” a correlation with the mechanical and chemical properties of films is developed. The following cell lines have been studied: mouse 3T3 and L929 fibroblasts, human foreskin primary fibroblasts VH-Fib, human embryonic kidney HEK-293, human bone cell line U-2-OS, Chinese hamster ovary CHO-K and mouse embryonic stem cells. All cells adhere and spread well in a narrow “cell-friendly” window identify in the range of n = 12–15. At n < 12, the film is inhomogeneous and at n > 15, the film is cell repellent for all cell lines. Cellular adhesion correlates with the mechanical properties of the films showing that softer films at higher “n” number exhibiting a significant decrease of the Young's modulus below 100 kPa are weakly adherent to cells. This trend cannot be reversed even by coating a strong cell-adhesive protein fibronectin onto the film. This indicates that mechanical cues plays a major role for cell behavior, also in respect to biochemical ones.
Authors with CRIS profile
Involved external institutions
Max-Planck-Institut für Kolloid- und Grenzflächenforschung / Max Planck Institute of Colloids and Interfaces
Germany (DE)
Fraunhofer-Institut für Zelltherapie und Immunologie (IZI)
Germany (DE)
Nottingham Trent University
United Kingdom (GB)
Germany (DE)
Fraunhofer-Institut für Zelltherapie und Immunologie (IZI)
Germany (DE)
Nottingham Trent University
United Kingdom (GB)
How to cite
APA:
Madaboosi, N., Uhlig, K., Schmidt, S., Vikulina, A.S., Moehwald, H., Duschl, C., & Volodkin, D. (2018). A “Cell-Friendly” Window for the Interaction of Cells with Hyaluronic Acid/Poly-l-Lysine Multilayers. Macromolecular Bioscience, 18(2). https://dx.doi.org/10.1002/mabi.201700319
MLA:
Madaboosi, Narayanan, et al. "A “Cell-Friendly” Window for the Interaction of Cells with Hyaluronic Acid/Poly-l-Lysine Multilayers." Macromolecular Bioscience 18.2 (2018).
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