Intravital quantification reveals dynamic calcium concentration changes across B cell differentiation stages

Ulbricht C, Leben R, Rakhymzhan A, Kirchhoff F, Nitschke L, Radbruch H, Niesner RA, Hauser AE (2021)


Publication Type: Journal article

Publication year: 2021

Journal

Book Volume: 10

Article Number: e56020

DOI: 10.7554/eLife.56020

Abstract

Calcium is a universal second messenger present in all eukaryotic cells. The mobilization and storage of Ca2+ ions drives a number of signaling-related processes, stress-responses or metabolic changes, all of which are relevant for the development of immune cells and their adaption to pathogens. Here, we introduce the FRET-reporter mouse YellowCaB expressing the genetically encoded calcium indicator TN-XXL in B lymphocytes. Calcium-induced conformation change of TN-XXL results in FRET-donor quenching measurable by two-photon fluorescence lifetime imaging. For the first time, using our novel numerical analysis, we extract absolute cytoplasmic calcium concentrations in activated B cells during affinity maturation in vivo. We show that calcium in activated B cells is highly dynamic and that activation introduces a persistent calcium heterogeneity to the lineage. A characterization of absolute calcium concentrations present at any time within the cytosol is therefore of great value for the understanding of long-lived beneficial and detrimental (auto)immunity.

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APA:

Ulbricht, C., Leben, R., Rakhymzhan, A., Kirchhoff, F., Nitschke, L., Radbruch, H.,... Hauser, A.E. (2021). Intravital quantification reveals dynamic calcium concentration changes across B cell differentiation stages. eLife, 10. https://dx.doi.org/10.7554/eLife.56020

MLA:

Ulbricht, Carolin, et al. "Intravital quantification reveals dynamic calcium concentration changes across B cell differentiation stages." eLife 10 (2021).

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