Silk fiber-reinforced hyaluronic acid-based hydrogel for cartilage tissue engineering
Weitkamp JT, Wöltje M, Nußpickel B, Schmidt FN, Aibibu D, Bayer A, Eglin D, Armiento AR, Arnold P, Cherif C, Lucius R, Smeets R, Kurz B, Behrendt P (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 22
Article Number: 3635
Journal Issue: 7
DOI: 10.3390/ijms22073635
Abstract
A continuing challenge in cartilage tissue engineering for cartilage regeneration is the creation of a suitable synthetic microenvironment for chondrocytes and tissue regeneration. The aim of this study was to develop a highly tunable hybrid scaffold based on a silk fibroin matrix (SM) and a hyaluronic acid (HA) hydrogel. Human articular chondrocytes were embedded in a porous 3-dimensional SM, before infiltration with tyramine modified HA hydrogel. Scaffolds were cultured in chondropermissive medium with and without TGF-β1. Cell viability and cell distribution were assessed using CellTiter-Blue assay and Live/Dead staining. Chondrogenic marker expression was detected using qPCR. Biosynthesis of matrix compounds was analyzed by dimethylmethylene blue assay and immuno-histology. Differences in biomaterial stiffness and stress relaxation were characterized using a one-step unconfined compression test. Cell morphology was investigated by scanning electron microscopy. Hybrid scaffold revealed superior chondro-inductive and biomechanical properties compared to sole SM. The presence of HA and TGF-β1 increased chondrogenic marker gene expression and matrix deposition. Hybrid scaffolds offer cytocompatible and highly tunable properties as cell-carrier systems, as well as favorable biomechanical properties.
Authors with CRIS profile
Involved external institutions
Christian-Albrechts-Universität zu Kiel
Germany (DE)
Université Jean Monnet Saint-Étienne
France (FR)
Universitätsklinikum Hamburg-Eppendorf (UKE)
Germany (DE)
Universitätsklinikum Schleswig-Holstein (UKSH)
Germany (DE)
AO Foundation
Switzerland (CH)
Germany (DE)
Université Jean Monnet Saint-Étienne
France (FR)
Universitätsklinikum Hamburg-Eppendorf (UKE)
Germany (DE)
Universitätsklinikum Schleswig-Holstein (UKSH)
Germany (DE)
AO Foundation
Switzerland (CH)
How to cite
APA:
Weitkamp, J.T., Wöltje, M., Nußpickel, B., Schmidt, F.N., Aibibu, D., Bayer, A.,... Behrendt, P. (2021). Silk fiber-reinforced hyaluronic acid-based hydrogel for cartilage tissue engineering. International Journal of Molecular Sciences, 22(7). https://doi.org/10.3390/ijms22073635
MLA:
Weitkamp, Jan Tobias, et al. "Silk fiber-reinforced hyaluronic acid-based hydrogel for cartilage tissue engineering." International Journal of Molecular Sciences 22.7 (2021).
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