Lineage-associated connexin 43 expression in bisphosphonate-exposed rat bones

Preidl R, Amann KU, Weber M, Schiller M, Ringler M, Ries J, Neukam FW, Kesting MR, Geppert CI, Wehrhan F (2021)

Publication Type: Journal article

Publication year: 2021

Journal

Journal of Cranio-Maxillofacial Surgery Elsevier

DOI: 10.1016/j.jcms.2021.02.010

Abstract

Expression of signaling proteins in bone cells depends on their embryological mesoderm-derived (e.g. tibia) or cranial neural crest (CNC)-derived (e.g. jaw) origin. Connexin 43 (Cx43) is a gap junction protein that plays an essential role in the mode of action of bisphosphonates (BP). This study aimed to investigate Cx43 expression and the influence of BP application on mesoderm- and CNC-derived bone. Using a rat model, molar extraction and tibia osteotomy with (Group 4) or without (Group 3) previous BP application was performed. Untreated (Group 1) and animals selectively treated with BPs (Group 2) served as controls. Cx43 expression was immunohistochemically determined 12 and 16 weeks postoperatively via a labeling index. Cx43 expression in CNC-derived bone was significantly higher compared with mesodermal bone. BP application decreased Cx43 expression; however, detected expression levels were still higher in jawbone (Group 2 tibia vs jaw: 5.83 ± 5.06 vs 23.52 ± 6.42; p = 0.007). During bone healing after surgical intervention (Group 3) there were no expression differences between tibia and jawbone. BP treatment prior to surgery resulted in significantly lower Cx43 expression in CNC-derived compared with tibia bone (Group 4 tibia vs jaw: 56.84 ± 15.57 vs 16.40 ± 5.66; p < 0.01). Increased Cx43 expression in jaw compared with tibia bone is in line with their embryological origins. A significant Cx43 suppression in jawbone after BP application and surgery might contribute to the selectively altered osseous turnover and development of MRONJ in CNC-derived bone.

Authors with CRIS profile

Raimund Preidl Department of Oral and Cranio-Maxillofacial Surgery Kerstin Ute Amann Professur für Nephropathologie Manuel Weber Department of Oral and Cranio-Maxillofacial Surgery Martin Schiller Department of Oral and Cranio-Maxillofacial Surgery Manuela Ringler Department of Oral and Cranio-Maxillofacial Surgery Jutta Ries Medizinische Fakultät Friedrich Wilhelm Neukam Medizinische Fakultät Marco Rainer Kesting Lehrstuhl für Zahnmedizin, insbesondere Mund-, Kiefer-, und Gesichtschirurgie Carol-Immanuel Geppert Institute of Pathology Falk Wehrhan Department of Oral and Cranio-Maxillofacial Surgery

How to cite

APA:

Preidl, R., Amann, K.U., Weber, M., Schiller, M., Ringler, M., Ries, J.,... Wehrhan, F. (2021). Lineage-associated connexin 43 expression in bisphosphonate-exposed rat bones. Journal of Cranio-Maxillofacial Surgery. https://doi.org/10.1016/j.jcms.2021.02.010

MLA:

Preidl, Raimund, et al. "Lineage-associated connexin 43 expression in bisphosphonate-exposed rat bones." Journal of Cranio-Maxillofacial Surgery (2021).

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