Preidl R, Reuß S, Neukam FW, Kesting MR, Wehrhan F (2021)
Publication Type: Journal article
Publication year: 2021
DOI: 10.1016/j.jcms.2021.02.006
The aim of this study was to investigate intraluminal vessel diameters and endothelial expression levels of pro-inflammatory and -thrombotic mediators in patent and non-patent microvascular anastomoses. Endothelial expression of CD31, VCAM-1, E− and P-Selectin, eNOS, iNOS and PAI-1 was evaluated by immunohistochemistry and compared to non-anastomosed arteries as controls. Intraluminal diameters were determined via H.E.-staining. In 20 human anastomoses (8 patent, 12 non-patent) neither the analysis of endoluminal de-endothelialization (p = 0.966) nor luminal narrowing (p = 0.750) revealed any significant differences between patent and non-patent microanastomoses. Expressions of pro-inflammatory mediators were significantly higher in patent anastomoses compared to controls but did not show any difference compared to non-patent anastomoses (p > 0.050). iNOS was higher in non-patent compared to patent anastomoses (p = 0.030) and controls (p = 0.001), whereas eNOS did not reveal any differences between these groups (p = 0.611 and p = 0.130). In non-patent anastomoses PAI-1 was expressed higher compared to patent anastomoses and controls (p = 0.021 and p < 0.001). Irrespective of their patency, anastomoses are characterized by endothelial dysfunction with a pro-inflammatory and pro-thrombotic milieu. Avoiding endothelial trauma during suturing is essential in order not to aggravate existing endothelial dysfunction in microanastomoses. Additionally, the influence of medication-related changes on anastomoses should be investigated as this is still an indistinctive topic.
APA:
Preidl, R., Reuß, S., Neukam, F.W., Kesting, M.R., & Wehrhan, F. (2021). Endothelial inflammatory and thrombogenic expression changes in microvascular anastomoses – An immunohistochemical analysis. Journal of Cranio-Maxillofacial Surgery. https://dx.doi.org/10.1016/j.jcms.2021.02.006
MLA:
Preidl, Raimund, et al. "Endothelial inflammatory and thrombogenic expression changes in microvascular anastomoses – An immunohistochemical analysis." Journal of Cranio-Maxillofacial Surgery (2021).
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