Optical diagnosis of oral cavity lesions by label-free Raman spectroscopy

Matthies L, Gebrekidan M, Tegtmeyer JF, Oetter N, Rohde M, Vollkommer T, Smeets R, Wilczak W, Stelzle F, Gosau M, Braeuer AS, Knipfer C (2021)

Publication Type: Journal article

Publication year: 2021


Book Volume: 12

Pages Range: 836-851

Journal Issue: 2

DOI: 10.1364/BOE.409456


Oral squamous cell carcinoma (OSCC) is one of the most prevalent cancers and frequently preceded by non-malignant lesions. Using Shifted-Excitation Raman Difference Spectroscopy (SERDS), principal component and linear discriminant analysis in native tissue specimens, 9500 raw Raman spectra of OSCC, 4300 of non-malignant lesions and 4200 of physiological mucosa were evaluated. Non-malignant lesions were distinguished from physiological mucosa with a classification accuracy of 95.3% (95.4% sensitivity, 95.2% specificity, area under the curve (AUC) 0.99). Discriminating OSCC from non-malignant lesions showed an accuracy of 88.4% (93.7% sensitivity, 76.7% specificity, AUC 0.93). OSCC was identified against physiological mucosa with an accuracy of 89.8% (93.7% sensitivity, 81.0% specificity, AUC 0.90). These findings underline the potential of SERDS for the diagnosis of oral cavity lesions.

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Matthies, L., Gebrekidan, M., Tegtmeyer, J.F., Oetter, N., Rohde, M., Vollkommer, T.,... Knipfer, C. (2021). Optical diagnosis of oral cavity lesions by label-free Raman spectroscopy. Biomedical Optics Express, 12(2), 836-851. https://dx.doi.org/10.1364/BOE.409456


Matthies, Levi, et al. "Optical diagnosis of oral cavity lesions by label-free Raman spectroscopy." Biomedical Optics Express 12.2 (2021): 836-851.

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