Inflammation in the human periodontium induces downregulation of the α1-and β1-subunits of the sgc in cementoclasts

Korkmaz Y, Puladi B, Galler K, Kämmerer PW, Schröder A, Gölz L, Sparwasser T, Bloch W, Friebe A, Deschner J (2021)

Publication Type: Journal article

Publication year: 2021

Journal

International Journal of Molecular Sciences MDPI

Book Volume: 22

Pages Range: 1-13

Article Number: 539

Journal Issue: 2

DOI: 10.3390/ijms22020539

Abstract

Nitric oxide (NO) binds to soluble guanylyl cyclase (sGC), activates it in a reduced oxidized heme iron state, and generates cyclic Guanosine Monophosphate (cGMP), which results in vasodilatation and inhibition of osteoclast activity. In inflammation, sGC is oxidized and becomes insensitive to NO. NO-and heme-independent activation of sGC requires protein expression of the α1-and β1-subunits. Inflammation of the periodontium induces the resorption of cementum by cementoclasts and the resorption of the alveolar bone by osteoclasts, which can lead to tooth loss. As the presence of sGC in cementoclasts is unknown, we investigated the α1-and β1-subunits of sGC in cementoclasts of healthy and inflamed human periodontium using double immunostaining for CD68 and cathepsin K and compared the findings with those of osteoclasts from the same sections. In comparison to cementoclasts in the healthy periodontium, cementoclasts under inflammatory conditions showed a decreased staining intensity for both α1-and β1-subunits of sGC, indicating reduced protein expression of these subunits. Therefore, pharmacological activation of sGC in inflamed periodontal tissues in an NO-and heme-independent manner could be considered as a new treatment strategy to inhibit cementum resorption.

Authors with CRIS profile

Lina Gölz Lehrstuhl für Zahn-, Mund- und Kieferheilkunde, insbesondere Kieferorthopädie

Involved external institutions

Johannes Gutenberg-Universität Mainz (JGU) DE Germany (DE) Universitätsklinikum Aachen (UKA) DE Germany (DE) Universitätsklinikum Regensburg DE Germany (DE) Universitätsmedizin der Johannes Gutenberg-Universität Mainz DE Germany (DE) Deutsche Sporthochschule Köln DE Germany (DE) Julius-Maximilians-Universität Würzburg DE Germany (DE)

How to cite

APA:

Korkmaz, Y., Puladi, B., Galler, K., Kämmerer, P.W., Schröder, A., Gölz, L.,... Deschner, J. (2021). Inflammation in the human periodontium induces downregulation of the α1-and β1-subunits of the sgc in cementoclasts. International Journal of Molecular Sciences, 22(2), 1-13. https://doi.org/10.3390/ijms22020539

MLA:

Korkmaz, Yüksel, et al. "Inflammation in the human periodontium induces downregulation of the α1-and β1-subunits of the sgc in cementoclasts." International Journal of Molecular Sciences 22.2 (2021): 1-13.

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