Gas plasma irradiation of breast cancers promotes immunogenicity, tumor reduction, and an abscopal effect in vivo
Mahdikia H, Saadati F, Freund E, Gaipl U, Majidzadeh-a K, Shokri B, Bekeschus S (2021)
Publication Type: Journal article
Publication year: 2021
Journal
Book Volume: 10
Article Number: 1859731
Journal Issue: 1
DOI: 10.1080/2162402X.2020.1859731
Abstract
While many new and emerging therapeutic concepts have appeared throughout the last decades, cancer still is fatal in many patients. At the same time, the importance of immunology in oncotherapy is increasingly recognized, not only since the advent of checkpoint therapy. Among the many types of tumors, also breast cancer has an immunological dimension that might be exploited best by increasing the immunogenicity of the tumors in the microenvironment. To this end, we tested a novel therapeutic concept, gas plasma irradiation, for its ability to promote the immunogenicity and increase the toxicity of breast cancer cells in vitro and in vivo. Mechanistically, this emerging medical technology is employing a plethora of reactive oxygen species being deposited on the target cells and tissues. Using 2D cultures and 3D tumor spheroids, we found gas plasma-irradiation to drive apoptosis and immunogenic cancer cell death (ICD) in vitro, as evidenced by an increased expression of calreticulin, heat-shock proteins 70 and 90, and MHC-I. In 4T1 breast cancer-bearing mice, the gas plasma irradiation markedly decreased tumor burden and increased survival. Interestingly, non-treated tumors injected in the opposite flank of mice exposed to our novel treatment also exhibited reduced growth, arguing for an abscopal effect. This was concomitant with an increase of apoptosis and tumor-infiltrating CD4+ and CD8+ T-cells as well as dendritic cells in the tissues. In summary, we found gas plasma-irradiated murine breast cancers to induce toxicity and augmented immunogenicity, leading to reduced tumor growth at a site remote to the treatment area.
Authors with CRIS profile
Involved external institutions
Shahid Beheshti University of Medical Sciences
Iran, Islamic Republic of (IR)
Leibniz-Institut für Plasmaforschung und Technologie e.V. (INP Greifswald) / Leibniz Institute for Plasma Science and Technology
Germany (DE)
ACECR Academic Center for Education, Culture and Research
Iran, Islamic Republic of (IR)
Iran, Islamic Republic of (IR)
Leibniz-Institut für Plasmaforschung und Technologie e.V. (INP Greifswald) / Leibniz Institute for Plasma Science and Technology
Germany (DE)
ACECR Academic Center for Education, Culture and Research
Iran, Islamic Republic of (IR)
How to cite
APA:
Mahdikia, H., Saadati, F., Freund, E., Gaipl, U., Majidzadeh-a, K., Shokri, B., & Bekeschus, S. (2021). Gas plasma irradiation of breast cancers promotes immunogenicity, tumor reduction, and an abscopal effect in vivo. OncoImmunology, 10(1). https://doi.org/10.1080/2162402X.2020.1859731
MLA:
Mahdikia, Hamed, et al. "Gas plasma irradiation of breast cancers promotes immunogenicity, tumor reduction, and an abscopal effect in vivo." OncoImmunology 10.1 (2021).
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