Defining metaniches in the oral cavity according to their microbial composition and cytokine profile

Seidel CL, Gerlach R, Wiedemann P, Weider M, Rodrian G, Hader M, Frey B, Gaipl U, Bozec A, Cieplik F, Kirschneck C, Bogdan C, Gölz L (2020)

Publication Type: Journal article

Publication year: 2020

Journal

International Journal of Molecular Sciences MDPI

Book Volume: 21

Pages Range: 1-22

Article Number: 8218

Journal Issue: 21

DOI: 10.3390/ijms21218218

Abstract

The human oral microbiota consists of over 700 widespread taxa colonizing the oral cavity in several anatomically diverse oral niches. Lately, sequencing of the 16S rRNA genes has become an acknowledged, culture-independent method to characterize the oral microbiota. However, only a small amount of data are available concerning microbial differences between oral niches in periodontal health and disease. In the context of periodontitis, the cytokine expression in the gingival crevicular fluid has been studied in detail, whereas little is known about the cytokine profile in hard and soft tissue biofilms. In order to characterize oral niches in periodontal health, the oral microbiota and cytokine pattern were analyzed at seven different sites (plaque (P), gingival crevicular fluid (GCF), saliva (S), tongue (T), hard palate (HP), cheek (C) and sublingual area (U)) of 20 young adults using next-generation sequencing and multiplex immunoassays. Site-specific microbial compositions were detected, which clustered into three distinct metaniches (“P-GCF”, “S-T-HP” and “C-U”) and were associated with niche-/metaniche-specific cytokine profiles. Our findings allow the definition of distinct metaniches according to their microbial composition, partly reflected by their cytokine profile, and provide new insights into microenvironmental similarities between anatomical diverse oral niches.

Authors with CRIS profile

Roman Gerlach Institute of Microbiology – Clinical Microbiology, Immunology and Hygiene Matthias Weider Department of Orthodontics and Orofacial Orthopaedics Michael Hader Department of Radiation Oncology Benjamin Frey Department of Radiation Oncology Udo Gaipl Department of Radiation Oncology Aline Bozec Professur für Experimentelle Immuntherapie Christian Bogdan Lehrstuhl für Mikrobiologie und Infektionsimmunologie Lina Gölz Lehrstuhl für Zahn-, Mund- und Kieferheilkunde, insbesondere Kieferorthopädie

Involved external institutions

Universitätsklinikum Regensburg DE Germany (DE)

How to cite

APA:

Seidel, C.L., Gerlach, R., Wiedemann, P., Weider, M., Rodrian, G., Hader, M.,... Gölz, L. (2020). Defining metaniches in the oral cavity according to their microbial composition and cytokine profile. International Journal of Molecular Sciences, 21(21), 1-22. https://doi.org/10.3390/ijms21218218

MLA:

Seidel, Corinna Lesley, et al. "Defining metaniches in the oral cavity according to their microbial composition and cytokine profile." International Journal of Molecular Sciences 21.21 (2020): 1-22.

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