Knockdown of the hypertension-associated gene NOSTRIN alters glomerular barrier function in zebrafish (Danio rerio).

Kirsch T, Kaufeld J, Korstanje R, Hentschel DM, Staggs L, Bollig F, Beese M, Schroder P, Boehme L, Haller H, Schiffer M (2013)

Publication Type: Journal article

Publication year: 2013

Journal

Hypertension American Heart Association

Book Volume: 62

Pages Range: 726-730

Journal Issue: 4

DOI: 10.1161/HYPERTENSIONAHA.113.01882

Abstract

Hypertension is one of the major risk factors for chronic kidney disease. Using quantitative trait loci analysis, we identified the gene of the F-BAR protein NOSTRIN in the center of an overlapping region in rat and human quantitative trait loci that are associated with hypertension. Immunohistochemical analysis revealed a predominantly podocytic expression pattern of NOSTRIN in human and mouse glomeruli. Further, NOSTRIN colocalizes with cell-cell contact-associated proteins β-catenin and zonula occludens-1 and interacts with the slit-membrane-associated adaptor protein CD2AP. In zebrafish larvae, knockdown of nostrin alters the glomerular filtration barrier function, inducing proteinuria and leading to ultrastructural morphological changes on the endothelial and epithelial side and of the glomerular basement membrane of the glomerular capillary loop. We conclude that NOSTRIN expression is an important factor for the integrity of the glomerular filtration barrier. Disease-related alteration of NOSTRIN expression may not only affect the vascular endothelium and, therefore, contribute to endothelial cell dysfunction but might also contribute to the development of podocyte disease and proteinuria.

Authors with CRIS profile

Mario Schiffer

Involved external institutions

Medizinische Hochschule Hannover (MHH) / Hannover Medical School DE Germany (DE) Brigham and Women's Hospital (BWH) US United States (USA) (US) Howard Hughes Medical Institute US United States (USA) (US)

How to cite

APA:

Kirsch, T., Kaufeld, J., Korstanje, R., Hentschel, D.M., Staggs, L., Bollig, F.,... Schiffer, M. (2013). Knockdown of the hypertension-associated gene NOSTRIN alters glomerular barrier function in zebrafish (Danio rerio). Hypertension, 62(4), 726-730. https://doi.org/10.1161/HYPERTENSIONAHA.113.01882

MLA:

Kirsch, Torsten, et al. "Knockdown of the hypertension-associated gene NOSTRIN alters glomerular barrier function in zebrafish (Danio rerio)." Hypertension 62.4 (2013): 726-730.

BibTeX: Download