Histological correlates of hippocampal magnetization transfer images in drug-resistant temporal lobe epilepsy patients
Peixoto-Santos JE, Velasco TR, Carlotti CG, Assirati JA, Rezende GHdSe, Kobow K, Coras R, Blümcke I, Salmon CEG, Santos ACd, Leite JP (2020)
Publication Type: Journal article
Publication year: 2020
Journal
Book Volume: 28
Article Number: 102463
DOI: 10.1016/j.nicl.2020.102463
Abstract
Objective: Temporal lobe epilepsy patients (TLE) often present with hippocampal atrophy, increased T2 relaxation, and reduced magnetization transfer ratio (MTR) in magnetic resonance images (MRI). The histological correlates of the reduced hippocampal MTR are so far unknown. Since MTR is dependent on the tissue's macromolecules, our aim was to evaluate the correlations between cellular populations, extracellular matrix molecules and the MTR in TLE patients. Methods: Patients with TLE (n = 26) and voluntaries (=20) were scanned in a 3 Tesla MRI scanner, and MTR images were calculated from 3DT1 sequences with magnetization pulse on resonance. Immunohistochemistry for neurons, reactive astrocytes, activated microglia, and extracellular matrix chondroitin sulfate were performed in formalin fixed, paraffin embedded tissues of TLE and autopsy controls (n = 10). Results were considered significant with adjusted p < 0.05. Results: Compared to the respective controls, TLE patients had reduced hippocampal MTR, increased reactive astrocytes and activated microglia, increased extracellular chondroitin sulfate, and reduced neuron density, compares to controls. MTR correlated positively with neuron density in CA3 and with chondroitin sulfate in CA3 and CA1. Multiple linear regressions reinforced the correlations between chondroitin sulfate and MTR. Significance: Our data indicate that extracellular matrix molecules are the most significant histological correlates of magnetization transfer ratio in the hippocampus of TLE patients.
Authors with CRIS profile
Katja Kobow
Institute of Neuropathology
Roland Coras
Institute of Neuropathology
Ingmar Blümcke
Lehrstuhl für Neuropathologie
Involved external institutions
University of São Paulo / Universidade de São Paulo (USP)
Brazil (BR)
Federal University of São Paulo / Universidade Federal de São Paulo (UNIFESP)
Brazil (BR)
Universidade Federal de Minas Gerais (UFMG)
Brazil (BR)
Brazil (BR)
Federal University of São Paulo / Universidade Federal de São Paulo (UNIFESP)
Brazil (BR)
Universidade Federal de Minas Gerais (UFMG)
Brazil (BR)
How to cite
APA:
Peixoto-Santos, J.E., Velasco, T.R., Carlotti, C.G., Assirati, J.A., Rezende, G.H.d.S.e., Kobow, K.,... Leite, J.P. (2020). Histological correlates of hippocampal magnetization transfer images in drug-resistant temporal lobe epilepsy patients. NeuroImage: Clinical, 28. https://doi.org/10.1016/j.nicl.2020.102463
MLA:
Peixoto-Santos, Jose Eduardo, et al. "Histological correlates of hippocampal magnetization transfer images in drug-resistant temporal lobe epilepsy patients." NeuroImage: Clinical 28 (2020).
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