Mass Spectrometric Characterization of HSV-1 L-Particles From Human Dendritic Cells and BHK21 Cells and Analysis of Their Functional Role

Birzer A, Kraner M, Heilingloh CS, Mühl-Zürbes P, Hofmann J, Steinkasserer A, Popella L (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Book Volume: 11

Article Number: 1997

DOI: 10.3389/fmicb.2020.01997

Abstract

Herpes simplex virus type 1 (HSV-1) is a very common human pathogenic virus among the world’s population. The lytic replication cycle of HSV-1 is, amongst others, characterized by a tripartite viral gene expression cascade, the assembly of nucleocapsids involving their subsequent nuclear egress, tegumentation, re-envelopment and the final release of progeny viral particles. During productive infection of a multitude of different cell types, HSV-1 generates not only infectious heavy (H-) particles, but also non-infectious light (L-) particles, lacking the capsid. In monocyte-derived mature dendritic cells (mDCs), HSV-1 causes a non-productive infection with the predominant release of L-particles. Until now, the generation and function of L-particles is not well understood, however, they are described as factors transferring viral components to the cellular microenvironment. To obtain deeper insights into the L-particle composition, we performed a mass-spectrometry-based analysis of L-particles derived from HSV-1-infected mDCs or BHK21 cells and H-particles from the latter one. In total, we detected 63 viral proteins in both H- and L-particle preparations derived from HSV-1-infected BHK21 cells. In L-particles from HSV-1-infected mDCs we identified 41 viral proteins which are differentially distributed compared to L-particles from BHK21 cells. In this study, we present data suggesting that L-particles modify mDCs and suppress their T cell stimulatory capacity. Due to the plethora of specific viral proteins incorporated into and transmitted by L-particles, it is tempting to speculate that L-particles manipulate non-infected bystander cells for the benefit of the virus.

Authors with CRIS profile

Involved external institutions

Universität Duisburg-Essen (UDE) Germany (DE)

How to cite

APA:

Birzer, A., Kraner, M., Heilingloh, C.S., Mühl-Zürbes, P., Hofmann, J., Steinkasserer, A., & Popella, L. (2020). Mass Spectrometric Characterization of HSV-1 L-Particles From Human Dendritic Cells and BHK21 Cells and Analysis of Their Functional Role. Frontiers in Microbiology, 11. https://doi.org/10.3389/fmicb.2020.01997

MLA:

Birzer, Alexandra, et al. "Mass Spectrometric Characterization of HSV-1 L-Particles From Human Dendritic Cells and BHK21 Cells and Analysis of Their Functional Role." Frontiers in Microbiology 11 (2020).

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