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Helicobacter pylori induce neutrophil transendothelial migration: Role of the bacterial HP-NAP

Brisslert M, Enarsson K, Lundin S, Karlsson A, Kusters JG, Svennerholm AM, Backert S, Quiding-Järbrink M (2005)

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Publication Type: Journal article

Publication year: 2005

Journal

Book Volume: 249

Pages Range: 95-103

Journal Issue: 1

DOI: 10.1016/j.femsle.2005.06.008

Abstract

Continuous recruitment of neutrophils into the inflamed gastric mucosal tissue is a hallmark of Helicobacter pylori infection in humans. In this study, we examined the ability of H. pylori to induce transendothelial migration of neutrophils using a transwell system consisting of a cultured monolayer of human endothelial cells as barrier between two chambers. We showed for the first time that live H. pylori, but not formalin-killed bacteria, induced a significantly increased transendothelial migration of neutrophils. H. pylori conditioned culture medium also induced significantly increased transendothelial migration, whereas heat-inactivated culture filtrates had no effect, suggesting that the chemotactic factor was proteinaceous. Depletion of H. pylori-neutrophil activating protein (HP-NAP) from the culture filtrates resulted in significant reduction of the transmigration. Culture filtrates from isogenic HP-NAP deficient mutant bacteria also induced significantly less neutrophil migration than culture filtrates obtained from wild-type bacteria. HP-NAP did not induce endothelial cell activation, suggesting that HP-NAP acts directly on the neutrophils. In conclusion, our results demonstrate that secreted HP-NAP is one of the factors resulting in H. pylori induced neutrophil transendothelial migration. We propose that HP-NAP contributes to the continuous recruitment of neutrophils to the gastric mucosa of H. pylori infected individuals. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

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How to cite

APA:

Brisslert, M., Enarsson, K., Lundin, S., Karlsson, A., Kusters, J.G., Svennerholm, A.-M.,... Quiding-Järbrink, M. (2005). Helicobacter pylori induce neutrophil transendothelial migration: Role of the bacterial HP-NAP. FEMS Microbiology Letters, 249(1), 95-103. https://doi.org/10.1016/j.femsle.2005.06.008

MLA:

Brisslert, Mikael, et al. "Helicobacter pylori induce neutrophil transendothelial migration: Role of the bacterial HP-NAP." FEMS Microbiology Letters 249.1 (2005): 95-103.

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