Amotl2 links ve-cadherin to contractile actin fibres necessary for aortic lumen expansion

Hultin S, Zheng Y, Mojallal M, Vertuani S, Gentili C, Balland M, Milloud R, Belting HG, Affolter M, Helker CSM, Adams RH, Herzog W, Uhlen P, Majumdar A, Holmgren L (2014)

Publication Type: Journal article

Publication year: 2014

Journal

Nature Communications Nature Publishing Group: Nature Communications

Book Volume: 5

Article Number: 3743

DOI: 10.1038/ncomms4743

Abstract

The assembly of individual endothelial cells into multicellular tubes is a complex morphogenetic event in vascular development. Extracellular matrix cues and cell-cell junctional communication are fundamental to tube formation. Together they determine the shape of endothelial cells and the tubular structures that they ultimately form. Little is known regarding how mechanical signals are transmitted between cells to control cell shape changes during morphogenesis. Here we provide evidence that the scaffold protein amotL2 is needed for aortic vessel lumen expansion. Using gene inactivation strategies in zebrafish, mouse and endothelial cell culture systems, we show that amotL2 associates to the VE-cadherin adhesion complex where it couples adherens junctions to contractile actin fibres. Inactivation of amotL2 dissociates VE-cadherin from cytoskeletal tensile forces that affect endothelial cell shape. We propose that the VE-cadherin/amotL2 complex is responsible for transmitting mechanical force between endothelial cells for the coordination of cellular morphogenesis consistent with aortic lumen expansion and function. © 2014 Macmillan Publishers Limited. All rights reserved.

Authors with CRIS profile

Wiebke Herzog

Involved external institutions

Karolinska University Hospital / Karolinska Universitetssjukhuset SE Sweden (SE) Universität Basel CH Switzerland (CH) Université Joseph Fourier (UJF Grenoble 1) FR France (FR) Westfälische Wilhelms-Universität (WWU) Münster DE Germany (DE) Karolinska Institute SE Sweden (SE) Max-Planck-Institut für molekulare Biomedizin / Max Planck Institute for Molecular Biomedicine DE Germany (DE)

How to cite

APA:

Hultin, S., Zheng, Y., Mojallal, M., Vertuani, S., Gentili, C., Balland, M.,... Holmgren, L. (2014). Amotl2 links ve-cadherin to contractile actin fibres necessary for aortic lumen expansion. Nature Communications, 5. https://dx.doi.org/10.1038/ncomms4743

MLA:

Hultin, Sara, et al. "Amotl2 links ve-cadherin to contractile actin fibres necessary for aortic lumen expansion." Nature Communications 5 (2014).

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