Expression patterns and role of the CadF protein in Campylobacter jejuni and Campylobacter coli

Krause-Gruszczynska M, Van Alphen LB, Oyarzabal OA, Alter T, Hänel I, Schliephake A, König W, Van Putten JP, Konkel ME, Backert S (2007)

Publication Type: Journal article

Publication year: 2007

Journal

FEMS Microbiology Letters Oxford University Press

Book Volume: 274

Pages Range: 9-16

Journal Issue: 1

DOI: 10.1111/j.1574-6968.2007.00802.x

Abstract

Binding of Campylobacter jejuni and Campylobacter coli to host fibronectin is mediated by the 37 kDa outer membrane protein CadF. Immunoblot analysis of 58 C. jejuni and C. coli isolates of human and animal origin showed that CadF is expressed in every strain. In most C. jejuni isolates, a 37 kDa band (p37) and a less-prominent 32 kDa band (p32) reacted with the antibodies. In C. coli isolates, CadF was consistently larger with sizes of 39 kDa (p39) and 34 kDa (p34), respectively. PCR analysis and sequencing revealed the presence of a 39-bp insertion sequence in the cadF gene of C. coli strains, explaining the increased molecular size. Infection assays revealed that C. jejuni bound and invaded INT-407 epithelial cells much more efficiently than C. coli and that this difference was considerably reduced in isogenic cadF mutants. These results demonstrate that CadF is an important pathogenicity factor. The difference between CadF of C. jejuni and C. coli may potentially be exploited to discriminate these species in food and clinical specimens. © 2007 Federation of European Microbiological Societies.

Authors with CRIS profile

Steffen Backert

Involved external institutions

Universiteit Utrecht (UU) / Utrecht University NL Netherlands (NL) Washington State University (WSU) US United States (USA) (US) Otto-von-Guericke-Universität Magdeburg DE Germany (DE) Bundesinstitut für Risikobewertung (BfR) DE Germany (DE) Friedrich-Loeffler-Institut (FLI), Bundesforschungsinstitut für Tiergesundheit DE Germany (DE) Landesamt für Verbraucherschutz Sachsen-Anhalt DE Germany (DE) Auburn University US United States (USA) (US)

How to cite

APA:

Krause-Gruszczynska, M., Van Alphen, L.B., Oyarzabal, O.A., Alter, T., Hänel, I., Schliephake, A.,... Backert, S. (2007). Expression patterns and role of the CadF protein in Campylobacter jejuni and Campylobacter coli. FEMS Microbiology Letters, 274(1), 9-16. https://doi.org/10.1111/j.1574-6968.2007.00802.x

MLA:

Krause-Gruszczynska, Malgorzata, et al. "Expression patterns and role of the CadF protein in Campylobacter jejuni and Campylobacter coli." FEMS Microbiology Letters 274.1 (2007): 9-16.

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