Enteropathogenic escherichia coli subverts phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate upon epithelial cell infection
Sason H, Milgrom M, Weiss AM, Melamed-Book N, Balla T, Grinstein S, Backert S, Rosenshine I, Aroeti B (2009)
Publication Type: Journal article
Publication year: 2009
Journal
Book Volume: 20
Pages Range: 544-555
Journal Issue: 1
Abstract
Phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] and phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P3] are phosphoi-nositides (PIs) present in small amounts in the inner leaflet of the plasma membrane (PM) lipid bilayer of host target cells. They are thought to modulate the activity of proteins involved in enteropathogenic Escherichia coli (EPEC) infection. However, the role of PI(4,5)P2 and PI(3,4,5)P 3 in EPEC pathogenesis remains obscure. Here we show that EPEC induces a transient PI(4,5)P2 accumulation at bacterial infection sites. Simultaneous actin accumulation, likely involved in the construction of the actin-rich pedestal, is also observed at these sites. Acute PI(4,5)P2 depletion partially diminishes EPEC adherence to the cell surface and actin pedestal formation. These findings are consistent with a bimodal role, whereby PI(4,5)P2 contributes to EPEC association with the cell surface and to the maximal induction of actin pedestals. Finally, we show that EPEC induces PI(3,4,5)P3 clustering at bacterial infection sites, in a translocated intimin receptor (Tir)-dependent manner. Tir phosphorylated on tyrosine 454, but not on tyrosine 474, forms complexes with an active phosphatidylinositol 3-kinase (PI3K), suggesting that PI3K recruited by Tir prompts the production of PI(3,4,5)P3 beneath EPEC attachment sites. The functional significance of this event may be related to the ability of EPEC to modulate cell death and innate immunity.© 2008 by The American Society for Cell Biology.
Authors with CRIS profile
Involved external institutions
The Hospital for Sick Children (SickKids)
Canada (CA)
Hebrew University of Jerusalem
Israel (IL)
University College Dublin (UCD)
Ireland (IE)
Eunice Kennedy Shriver National Institute of Child Health and Human Development
United States (USA) (US)
Canada (CA)
Hebrew University of Jerusalem
Israel (IL)
University College Dublin (UCD)
Ireland (IE)
Eunice Kennedy Shriver National Institute of Child Health and Human Development
United States (USA) (US)
How to cite
APA:
Sason, H., Milgrom, M., Weiss, A.M., Melamed-Book, N., Balla, T., Grinstein, S.,... Aroeti, B. (2009). Enteropathogenic escherichia coli subverts phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate upon epithelial cell infection. Molecular Biology of the Cell, 20(1), 544-555. https://doi.org/10.1091/mbc.E08-05-0516
MLA:
Sason, Hagit, et al. "Enteropathogenic escherichia coli subverts phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate upon epithelial cell infection." Molecular Biology of the Cell 20.1 (2009): 544-555.
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