Comparison of hydrogels for the development of well-defined 3d cancer models of breast cancer and melanoma

Schmid R, Schmidt S, Hazur J, Detsch R, Maurer E, Boccaccini AR, Hauptstein J, Teßmar J, Blunk T, Schrüfer S, Schubert DW, Horch RE, Boßerhoff AK, Arkudas A, Kengelbach-Weigand A (2020)


Publication Language: English

Publication Type: Journal article

Publication year: 2020

Journal

Book Volume: 12

Pages Range: 1-21

Article Number: 2320

Journal Issue: 8

DOI: 10.3390/cancers12082320

Abstract

Bioprinting offers the opportunity to fabricate precise 3D tumor models to study tumor pathophysiology and progression. However, the choice of the bioink used is important. In this study, cell behavior was studied in three mechanically and biologically different hydrogels (alginate, alginate dialdehyde crosslinked with gelatin (ADA–GEL), and thiol-modified hyaluronan (HA-SH crosslinked with PEGDA)) with cells from breast cancer (MDA-MB-231 and MCF-7) and melanoma (Mel Im and MV3), by analyzing survival, growth, and the amount of metabolically active, living cells via WST-8 labeling. Material characteristics were analyzed by dynamic mechanical analysis. Cell lines revealed significantly increased cell numbers in low-percentage alginate and HA-SH from day 1 to 14, while only Mel Im also revealed an increase in ADA–GEL. MCF-7 showed a preference for 1% alginate. Melanoma cells tended to proliferate better in ADA– GEL and HA-SH than mammary carcinoma cells. In 1% alginate, breast cancer cells showed equally good proliferation compared to melanoma cell lines. A smaller area was colonized in high-percentage alginate-based hydrogels. Moreover, 3% alginate was the stiffest material, and 2.5% ADA–GEL was the softest material. The other hydrogels were in the same range in between. Therefore, cellular responses were not only stiffness-dependent. With 1% alginate and HA-SH, we identified matrices that enable proliferation of all tested tumor cell lines while maintaining expected tumor heterogeneity. By adapting hydrogels, differences could be accentuated. This opens up the possibility of understanding and analyzing tumor heterogeneity by biofabrication.

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How to cite

APA:

Schmid, R., Schmidt, S., Hazur, J., Detsch, R., Maurer, E., Boccaccini, A.R.,... Kengelbach-Weigand, A. (2020). Comparison of hydrogels for the development of well-defined 3d cancer models of breast cancer and melanoma. Cancers, 12(8), 1-21. https://doi.org/10.3390/cancers12082320

MLA:

Schmid, Rafael, et al. "Comparison of hydrogels for the development of well-defined 3d cancer models of breast cancer and melanoma." Cancers 12.8 (2020): 1-21.

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