Topical application of Chlorin e6-PVP (Ce6-PVP) for improved endoscopic detection of neoplastic lesions in a murine colitis-associated cancer model

Ganzleben I, Hohmann M, Grünberg A, Menezes JG, Liebing E, Günther C, Thonn V, Beß D, Becker C, Schmidt M, Neurath M, Waldner M, Vieth M (2020)

Publication Type: Journal article

Publication year: 2020

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Book Volume: 10

Article Number: 13129

Journal Issue: 1

DOI: 10.1038/s41598-020-69570-2

Abstract

Screening colonoscopy is crucial in reducing the mortality of colorectal cancer. However, detecting adenomas against the backdrop of an inflamed mucosa (e.g. in ulcerative colitis) remains exceedingly difficult. Therefore, we aimed to improve neoplastic lesion detection by employing a fluorescence-based endoscopic approach. We used the well-established murine AOM/DSS model to induce inflammation-driven carcinogenesis in the colon. In our diagnostic approach, we evaluated Chlorin e6 polyvinylpyrrolidone (Ce6-PVP)-based fluorescence endoscopy in comparison to standard white-light endoscopy. A specialized pathologist then analyzed the histology of the detected lesions. Complementary in vitro studies were performed using human cell lines and a murine organoid system. Ce6-PVP-based fluorescence endoscopy had an improved detection rate of 100% (8/8) in detecting high-grade dysplasias and carcinomas over white-light detection alone with 75% (6/8). Trade-off for this superior detection rate was an increased rate of false positive lesions with an increase in the false discovery rate from 45% for white-light endoscopy to 81% for fluorescence endoscopy. We demonstrate in a proof-of-concept study that Ce6-PVP-based fluorescence endoscopy is a highly sensitive red flag technology to identify biopsy-worthy lesions in the colon.

Authors with CRIS profile

Ingo Ganzleben Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Martin Hohmann Erlangen Graduate School in Advanced Optical Technologies (SAOT) Eva Liebing Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Claudia Günther Department of Medicine 1 – Gastroenterology, Pneumology and Endocrinology Christoph Becker Professur für Molekulare Gastroenterologie Michael Schmidt Lehrstuhl für Photonische Technologien (LPT) Markus Neurath Lehrstuhl für Innere Medizin I (Medizin 1) Maximilian Waldner Professur für Funktionelle Bildgebung in der Medizin Michael Vieth

Additional Organisation(s)

Erlangen Graduate School in Advanced Optical Technologies (SAOT)

Involved external institutions

Klinikum Bayreuth DE Germany (DE)

How to cite

APA:

Ganzleben, I., Hohmann, M., Grünberg, A., Menezes, J.G., Liebing, E., Günther, C.,... Vieth, M. (2020). Topical application of Chlorin e6-PVP (Ce6-PVP) for improved endoscopic detection of neoplastic lesions in a murine colitis-associated cancer model. Scientific Reports, 10(1). https://doi.org/10.1038/s41598-020-69570-2

MLA:

Ganzleben, Ingo, et al. "Topical application of Chlorin e6-PVP (Ce6-PVP) for improved endoscopic detection of neoplastic lesions in a murine colitis-associated cancer model." Scientific Reports 10.1 (2020).

BibTeX: Download