Differential gene expression in colon carcinoma cells and tissues detected with a cDNA array

Backert S, Gelos M, Kobalz U, Hanski ML, Böhm C, Mann B, Lövin N, Gratchev A, Mansmann U, Moyer MP, Riecken EO, Hanski C (1999)

Publication Type: Journal article

Publication year: 1999

Journal

International Journal of Cancer Wiley-Blackwell

Book Volume: 82

Pages Range: 868-874

Journal Issue: 6

DOI: 10.1002/(SICI)1097-0215(19990909)82:6<868::AID-IJC16>3.0.CO;2-W

Abstract

Expression of selected genes coding for proteins with defined cellular functions was analysed in human cell lines derived from normal colonic mucosa, non-mucinous colonic carcinomas and mucinous colonic carcinomas. Altered expression of 10 genes in colon carcinoma cells was found by using a cDNA array; 6 of these alterations (60%) were confirmed by Northern blotting or semi-quantitative reverse transcription-polymerase chain reaction (RT- PCR). Among these 6 genes, 3 transcription factors as well as the topoisomerase II α and the mitosis inhibitor WEEIHu gene were significantly suppressed in the tumour cell lines. In addition, the gene coding for the cell cycle inhibitor p21 was overexpressed only in cell lines derived from mucinous carcinomas. The significant suppression of the kinase WEEIHu gene in carcinoma cells of both phenotypes and the tendency of the mucinous phenotype to overexpress p21 protein were confirmed in human colon carcinoma tissues. Our data show that the cDNA array method permits a correct identification of changes in gene expression with a relatively high accuracy. The different expression of the p21 gene in the non-mucinous and mucinous carcinoma cells supports the hypothesis that these phenotypes may develop along different genetic pathways. The detection of WEEIHu gene suppression in colon carcinoma cells and tissues suggests its potential role in tumourigenesis.

Authors with CRIS profile

Steffen Backert

Involved external institutions

Freie Universität Berlin DE Germany (DE) University of Texas at San Antonio US United States (USA) (US)

How to cite

APA:

Backert, S., Gelos, M., Kobalz, U., Hanski, M.L., Böhm, C., Mann, B.,... Hanski, C. (1999). Differential gene expression in colon carcinoma cells and tissues detected with a cDNA array. International Journal of Cancer, 82(6), 868-874. https://dx.doi.org/10.1002/(SICI)1097-0215(19990909)82:6<868::AID-IJC16>3.0.CO;2-W

MLA:

Backert, Steffen, et al. "Differential gene expression in colon carcinoma cells and tissues detected with a cDNA array." International Journal of Cancer 82.6 (1999): 868-874.

BibTeX: Download