cfNOMe - A single assay for comprehensive epigenetic analyses of cell-free DNA
Erger F, Nörling D, Borchert D, Leenen E, Habbig S, Wiesener MS, Bartram MP, Wenzel A, Becker C, Toliat MR, Nürnberg P, Beck BB, Altmüller J (2020)
Publication Type: Journal article
Publication year: 2020
Journal
Book Volume: 12
Pages Range: 54-
Journal Issue: 1
DOI: 10.1186/s13073-020-00750-5
Abstract
Cell-free DNA (cfDNA) analysis has become essential in cancer diagnostics and prenatal testing. We present cfNOMe, a two-in-one method of measuring cfDNA cytosine methylation and nucleosome occupancy in a single assay using non-disruptive enzymatic cytosine conversion and a custom bioinformatic pipeline. We show that enzymatic cytosine conversion better preserves cfDNA fragmentation information than does bisulfite conversion. Whereas previously separate experiments were required to study either epigenetic marking, cfNOMe delivers reliable results for both, enabling more comprehensive and inexpensive epigenetic cfDNA profiling. cfNOMe has the potential to advance biomarker discovery and diagnostic usage in diseases with systemic perturbations of cfDNA composition.
Involved external institutions
Universität Witten/Herdecke
Germany (DE)
Universitätsklinikum Köln
Germany (DE)
Universität zu Köln
Germany (DE)
Germany (DE)
Universitätsklinikum Köln
Germany (DE)
Universität zu Köln
Germany (DE)
How to cite
APA:
Erger, F., Nörling, D., Borchert, D., Leenen, E., Habbig, S., Wiesener, M.S.,... Altmüller, J. (2020). cfNOMe - A single assay for comprehensive epigenetic analyses of cell-free DNA. Genome Medicine, 12(1), 54-. https://dx.doi.org/10.1186/s13073-020-00750-5
MLA:
Erger, Florian, et al. "cfNOMe - A single assay for comprehensive epigenetic analyses of cell-free DNA." Genome Medicine 12.1 (2020): 54-.
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