Optical tomography complements light sheet microscopy for in toto imaging of zebrafish development

Bassi A, Schmid B, Huisken J (2015)

Publication Type: Journal article

Publication year: 2015

Journal

Development Company of Biologists

Book Volume: 142

Pages Range: 1016-1020

Journal Issue: 5

DOI: 10.1242/dev.116970

Abstract

Fluorescently labeled structures can be spectrally isolated and imaged at high resolution in living embryos by light sheet microscopy. Multimodal imaging techniques are now needed to put these distinct structures back into the context of the surrounding tissue.We found that the bright-field contrast of unstained specimens in a selective plane illumination microscopy (SPIM) setup can be exploited for in vivo tomographic reconstructions of the threedimensional anatomy of zebrafish, without causing phototoxicity. We report multimodal imaging of entire zebrafish embryos over several hours of development, as well as segmentation, tracking and automatic registration of individual organs.

Authors with CRIS profile

Benjamin Schmid

Involved external institutions

Max-Planck-Institut für molekulare Zellbiologie und Genetik / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) DE Germany (DE)

How to cite

APA:

Bassi, A., Schmid, B., & Huisken, J. (2015). Optical tomography complements light sheet microscopy for in toto imaging of zebrafish development. Development, 142(5), 1016-1020. https://dx.doi.org/10.1242/dev.116970

MLA:

Bassi, Andrea, Benjamin Schmid, and Jan Huisken. "Optical tomography complements light sheet microscopy for in toto imaging of zebrafish development." Development 142.5 (2015): 1016-1020.

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