Hildebrand J, Göen T (2020)
Publication Type: Journal article
Publication year: 2020
Book Volume: 61
Article Number: 126538
DOI: 10.1016/j.jtemb.2020.126538
Background: The urinary excreted selenium species selenosugar 1 (SeSug1) plays a key role for monitoring of supplemental selenium exposure, e.g. by occupational exposure. In order to reproduce its contents in the long term, the integrity of SeSug1 in the urine is essential. Studies on the stability of SeSug1 in urine samples stored at −20 °C have shown that degradation of SeSug 1 occurs, requiring adequate countermeasures. Methods: Here, we explored the long-term stability of SeSug1 under usual storage conditions at −20 °C. For this purpose, the simultaneous determination of selenosugar 1 and methylselenic acid (MeSeA) was used to explore the stabilizing of the SeSug1 content by applying sodium azide (NaN3) as a bactericide or/and 5 M ammonium acetate buffer for pH control. Results: In untreated urine, conversion of SeSug1 to MeSeA was evident within days. Differences in urine matrices clearly showed different impact, which could be attributed to different buffer strengths by the urine itself. For durability, various concentrations of sodium azide were first applied, followed by pH buffering. A combination of 0.1% NaN3 and pH of 5.5 kept the SeSug1 content stable for over 3 months. Conclusion: The formation of MeSeA as degradation product of SeSug1 could be confirmed. Based on the proportions, an oxidation-based decomposition pathway was proposed. The investigations revealed that the complex interaction of pH buffering and bactericidal activity must be taken into account in order to stabilize SeSug1 in the urine. The main effect was the addition of NaN3. However, the alkaline nature of NaN3 required a sufficient buffering of the urinary matrix at a pH of 5.5.
APA:
Hildebrand, J., & Göen, T. (2020). Instability of urinary excreted methyl-2-acetamido-2-deoxy-1-seleno-β-D-galactopyranoside (selenosugar 1), the main elimination product of human selenium metabolism, and measures for its stabilization. Journal of Trace Elements in Medicine and Biology, 61. https://dx.doi.org/10.1016/j.jtemb.2020.126538
MLA:
Hildebrand, Jörg, and Thomas Göen. "Instability of urinary excreted methyl-2-acetamido-2-deoxy-1-seleno-β-D-galactopyranoside (selenosugar 1), the main elimination product of human selenium metabolism, and measures for its stabilization." Journal of Trace Elements in Medicine and Biology 61 (2020).
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