Morphologic HIV demonstration in formalin embedded material--techniques, problems, results

Zietz C, Speiser B, Hell W, Stürzl M, Wolf H, Roth WK (1991)

Publication Status: Published

Publication Type: Journal article

Publication year: 1991

Journal

Verhandlungen der Deutschen Gesellschaft für Pathologie Elsevier BV

Book Volume: 75

Pages Range: 149-54

Abstract

Formalin-fixed, paraffin-embedded material of archival specimens is suitable for a morphological HIV-detection: Infected cells with HIV in the proliferative phase can be demonstrated with reliable results on tissue sections by immunohistological technics using new antibodies. In situ nucleic acid hybridisation technics can also show HIV in the expression phase on paraffin-embedded material, but often fail in demonstrating latently HIV-infected cells. The DNA-Polymerase chain reaction can detect latent Provirus in morphologically defined areas of paraffin sections even in autopsy material, i.e. lymphnodes and even eyes of patients with HIV-Infection, but requires precaution and control with respect to contamination.

Authors with CRIS profile

Michael Stürzl Professur für Molekulare und Experimentelle Chirurgie

Involved external institutions

Max-Planck-Institut für Biochemie / Max Planck Institute of Biochemistry DE Germany (DE) Universität Basel CH Switzerland (CH) Technische Universität München (TUM) DE Germany (DE) Universität Regensburg DE Germany (DE) Ruhr-Universität Bochum (RUB) DE Germany (DE)

How to cite

APA:

Zietz, C., Speiser, B., Hell, W., Stürzl, M., Wolf, H., & Roth, W.K. (1991). Morphologic HIV demonstration in formalin embedded material--techniques, problems, results. Verhandlungen der Deutschen Gesellschaft für Pathologie, 75, 149-54.

MLA:

Zietz, Christian, et al. "Morphologic HIV demonstration in formalin embedded material--techniques, problems, results." Verhandlungen der Deutschen Gesellschaft für Pathologie 75 (1991): 149-54.

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