Fibrin gel-immobilized VEGF and bFGF efficiently stimulate angiogenesis in the AV loop model.

Arkudas A, Tjiawi J, Bleiziffer O, Grabinger L, Polykandriotis E, Beier JP, Stürzl M, Horch RE, Kneser U (2007)

Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 2007

Journal

Molecular Medicine Feinstein Institute for Medical Research

Book Volume: 13

Pages Range: 480-7

Journal Issue: 9-10

DOI: 10.2119/2007-00057.Arkudas

Abstract

The modulation of angiogenic processes in matrices is of great interest in tissue engineering. We assessed the angiogenic effects of fibrin-immobilized VEGF and bFGF in an arteriovenous loop (AVL) model in 22 AVLs created between the femoral artery and vein in rats. The loops were placed in isolation chambers and were embedded in 500 microL fibrin gel (FG) (group A) or in 500 microL FG loaded with 0.1 ng/microL VEGF and 0.1 ng/microL bFGF (group B). After two and four weeks specimens were explanted and investigated using histological, morphometrical, and ultramorphological [scanning electron microscope (SEM) of vascular corrosion replicas] techniques. In both groups, the AVL induced formation of densely vascularized connective tissue with differentiated and functional vessels inside the fibrin matrix. VEGF and bFGF induced significantly higher absolute and relative vascular density and a faster resorption of the fibrin matrix. SEM analysis in both groups revealed characteristics of an immature vascular bed, with a higher vascular density in group B. VEGF and bFGF efficiently stimulated sprouting of blood vessels in the AVL model. The implantation of vascular carriers into given growth factor-loaded matrix volumes may eventually allow efficient generation of axially vascularized, tissue-engineered composites.

Authors with CRIS profile

Andreas Arkudas Medizinische Fakultät Elias Polykandriotis Medizinische Fakultät Michael Stürzl Professur für Molekulare und Experimentelle Chirurgie Raymund Horch Professur für Plastische Chirurgie und Handchirurgie

Involved external institutions

Rheinisch-Westfälische Technische Hochschule (RWTH) Aachen DE Germany (DE) Ruprecht-Karls-Universität Heidelberg DE Germany (DE)

How to cite

APA:

Arkudas, A., Tjiawi, J., Bleiziffer, O., Grabinger, L., Polykandriotis, E., Beier, J.P.,... Kneser, U. (2007). Fibrin gel-immobilized VEGF and bFGF efficiently stimulate angiogenesis in the AV loop model. Molecular Medicine, 13(9-10), 480-7. https://doi.org/10.2119/2007-00057.Arkudas

MLA:

Arkudas, Andreas, et al. "Fibrin gel-immobilized VEGF and bFGF efficiently stimulate angiogenesis in the AV loop model." Molecular Medicine 13.9-10 (2007): 480-7.

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