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Neuronal nicotinic receptor expression in sensory neurons of the rat trigeminal ganglion: Demonstration of alpha 3 beta 4, a novel subtype in the mammalian nervous system

Flores CM, DeCamp RM, Kilo S, Rogers SW, Hargreaves KM (1996)

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Publication Status: Published

Publication Type: Journal article, Original article

Publication year: 1996

Journal

Publisher: SOC NEUROSCIENCE

Book Volume: 16

Pages Range: 7892-7901

Journal Issue: 24

Abstract

The identification of a family of neuronal nicotinic receptor subunit genes establishes the potential for multiple subtypes with diverse physiological functions. Virtually all of the high affinity nicotinic receptors measured to date in the rodent CNS are composed of alpha 4 and beta 2 subunits only. However, the demonstration of other subunit transcripts in a variety of central and peripheral nervous tissues suggests a greater degree of receptor subtype heterogeneity than so far has been elucidated. The purpose of the present studies was to determine at the mRNA and protein levels which neuronal nicotinic receptor subunits are expressed by sensory neurons of the rat trigeminal ganglion and in what combinations these gene products associate to form neuronal nicotinic receptor subtypes in this tissue. Radioreceptor binding analysis indicated that in the adult rat trigeminal ganglion there exist at least two nicotinic receptor binding sites with differing affinities for [H-3]-epibatidine. In situ hybridization histochemical studies revealed the existence of mRNA encoding the alpha 3, alpha 4, alpha 5, beta 2, and beta 4 subunits, but not the alpha 2 subunit. Immunoprecipitation with subunit-specific antisera demonstrated that each of the subunits present in the ganglion at the mRNA level is a constituent of nicotinic receptors capable of binding H-3-epibatidine. Various applications of these approaches yielded strong evidence that, in addition to alpha 4 beta 2, which is thought to be the predominant neuronal nicotinic receptor subtype in the rodent CNS, trigeminal sensory neurons express as the principal subtype alpha 3 beta 4, which has not been demonstrated previously in mammalian nervous tissue.

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How to cite

APA:

Flores, C.M., DeCamp, R.M., Kilo, S., Rogers, S.W., & Hargreaves, K.M. (1996). Neuronal nicotinic receptor expression in sensory neurons of the rat trigeminal ganglion: Demonstration of alpha 3 beta 4, a novel subtype in the mammalian nervous system. Journal of Neuroscience, 16(24), 7892-7901.

MLA:

Flores, Christopher M., et al. "Neuronal nicotinic receptor expression in sensory neurons of the rat trigeminal ganglion: Demonstration of alpha 3 beta 4, a novel subtype in the mammalian nervous system." Journal of Neuroscience 16.24 (1996): 7892-7901.

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