Electrophoretic Deposition of Copper(II)-Chitosan Complexes for Antibacterial Coatings

Akhtar MA, Ilyas K, Dlouhý I, Siska F, Boccaccini AR (2020)

Publication Type: Journal article

Publication year: 2020


Book Volume: 21

Journal Issue: 7

DOI: 10.3390/ijms21072637


Bacterial infection associated with medical implants is a major threat to healthcare. This work reports the fabrication of Copper(II)-Chitosan (Cu(II)-CS) complex coatings deposited by electrophoretic deposition (EPD) as potential antibacterial candidate to combat microorganisms to reduce implant related infections. The successful deposition of Cu(II)-CS complex coatings on stainless steel was confirmed by physicochemical characterizations. Morphological and elemental analyses by scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) spectroscopy verified the uniform distribution of copper in the Chitosan (CS) matrix. Moreover, homogeneous coatings without precipitation of metallic copper were confirmed by X-ray diffraction (XRD) spectroscopy and SEM micrographs. Controlled swelling behavior depicted the chelation of copper with polysaccharide chains that is key to the stability of Cu(II)-CS coatings. All investigated systems exhibited stable degradation rate in phosphate buffered saline (PBS)-lysozyme solution within seven days of incubation. The coatings presented higher mechanical properties with the increase in Cu(II) concentration. The crack-free coatings showed mildly hydrophobic behavior. Antibacterial assays were performed using both Gram-positive and Gram-negative bacteria. Outstanding antibacterial properties of the coatings were confirmed. After 24 h of incubation, cell studies of coatings confirms that up to a certain threshold concentration of Cu(II) were not cytotoxic to human osteoblast-like cells. Overall, our results show that uniform and homogeneous Cu(II)-CS coatings with good antibacterial and enhanced mechanical stability could be successfully deposited by EPD. Such antibiotic-free antibacterial coatings are potential candidates for biomedical implants.

Authors with CRIS profile

Involved external institutions

How to cite


Akhtar, M.A., Ilyas, K., Dlouhý, I., Siska, F., & Boccaccini, A.R. (2020). Electrophoretic Deposition of Copper(II)-Chitosan Complexes for Antibacterial Coatings. International Journal of Molecular Sciences, 21(7). https://dx.doi.org/10.3390/ijms21072637


Akhtar, Muhammad Asim, et al. "Electrophoretic Deposition of Copper(II)-Chitosan Complexes for Antibacterial Coatings." International Journal of Molecular Sciences 21.7 (2020).

BibTeX: Download