Is ovarian tissue transport at supra-zero temperatures compared to body temperature optimal for follicle survival?

Raffel N, Dittrich R, Orlowski P, Tischer H, Sӧder S, Erber R, Hoffmann I, Beckmann M, Lotz L (2020)

Publication Type: Journal article

Publication year: 2020

Journal

In Vivo International Institute of Anticancer Research (IIAR)

Book Volume: 34

Pages Range: 533-541

Journal Issue: 2

DOI: 10.21873/invivo.11805

Abstract

Background/Aim: Transportation of ovarian cortex prior to freezing is used clinically; however, basic investigations of ovarian storage are limited and the question remains what temperature is optimal for transport over long distances and time periods. The aim of this study was to evaluate the rate of follicular loss over various time periods under two different temperatures and assess whether ovarian follicle viability is affected following cryopreservation and thawing subsequent to the transportation of ovarian tissue. Materials and Methods: Pig ovaries were transported at 4oC (n=10) or at 38oC (n=10) prior to cryopreservation. At 0, 4, 12 and 24 h tissues were fixed for histological examination and a LIVE/DEAD Assay. At the same time-points ovarian tissues were cryopreserved and analysed after thawing. Results: Histological evaluation and LIVE/DEAD Assay of freshly transported ovarian tissue showed significantly better follicle survival at 4oC during transportation duration. In cryopreserved ovarian tissues the LIVE/DEAD Assay showed a significant difference in the number of intact and dead follicles at 24 h in favor of 4oC (p<0.05). Conclusion: Ovarian tissue transportation should be kept at a minimum to prevent potential damage.

Authors with CRIS profile

Nathalie Bleisinger Department of Obstetrics and Gynaecology Ralf Dittrich Professur für Experimentelle Reproduktionsmedizin Ramona Erber Institute of Pathology Matthias Beckmann Lehrstuhl für Geburtshilfe und Frauenheilkunde Laura Lotz Professur für Experimentelle Reproduktionsmedizin

Involved external institutions

Klinikum Coburg DE Germany (DE)

How to cite

APA:

Raffel, N., Dittrich, R., Orlowski, P., Tischer, H., Sӧder, S., Erber, R.,... Lotz, L. (2020). Is ovarian tissue transport at supra-zero temperatures compared to body temperature optimal for follicle survival? In Vivo, 34(2), 533-541. https://doi.org/10.21873/invivo.11805

MLA:

Raffel, Nathalie, et al. "Is ovarian tissue transport at supra-zero temperatures compared to body temperature optimal for follicle survival?" In Vivo 34.2 (2020): 533-541.

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