Chimeric FLS2 receptors reveal the basis for differential flagellin perception in arabidopsis and tomato

Mueller K, Bittel P, Chinchilla D, Jehle AK, Albert M, Boller T, Felix G (2012)


Publication Type: Journal article

Publication year: 2012

Journal

Book Volume: 24

Pages Range: 2213-2224

Journal Issue: 5

DOI: 10.1105/tpc.112.096073

Abstract

The flagellin receptor of Arabidopsis thaliana, At-FLAGELLIN SENSING2 (FLS2), has become a model for mechanistic and functional studies on plant immune receptors. Here, we started out with a comparison of At-FLS2 and the orthologous tomato (Solanum lycopersicum) receptor Sl-FLS2. Both receptors specifically responded to picomolar concentrations of the genuine flg22 ligand but proved insensitive to >106-fold higher concentrations of CLV3 peptides that have recently been reported as a second type of ligand for At-FLS2. At-FLS2 and Sl-FLS2 exhibit species-specific differences in the recognition of shortened or sequence-modified flg22 ligands. To map the sites responsible for these species-specific traits on the FLS2 receptors, we performed domain swaps, substituting subsets of the 28 leucine-rich repeats (LRRs) in At-FLS2 with the corresponding LRRs from Sl-FLS2. We found that the LRRs 7 to 10 of Sl-FLS2 determine the high affinity of Sl-FLS2 for the core part RINSAKDD of flg22. In addition, we discovered importance of the LRRs 19 to 24 for the responsiveness to C-terminally modified flagellin peptides. These results indicate that ligand perception in FLS2 is a complex molecular process that involves LRRs from both the outermost and innermost LRRs of the FLS2 ectodomain. © 2012 American Society of Plant Biologists. All rights reserved.

Authors with CRIS profile

Additional Organisation(s)

Involved external institutions

How to cite

APA:

Mueller, K., Bittel, P., Chinchilla, D., Jehle, A.K., Albert, M., Boller, T., & Felix, G. (2012). Chimeric FLS2 receptors reveal the basis for differential flagellin perception in arabidopsis and tomato. The Plant Cell, 24(5), 2213-2224. https://dx.doi.org/10.1105/tpc.112.096073

MLA:

Mueller, Katharina, et al. "Chimeric FLS2 receptors reveal the basis for differential flagellin perception in arabidopsis and tomato." The Plant Cell 24.5 (2012): 2213-2224.

BibTeX: Download