Tools and strategies to match peptide-ligand receptor pairs

Butenko MA, Wildhagen M, Albert M, Jehle A, Kalbacher H, Aalen RB, Felix G (2014)

Publication Type: Journal article

Publication year: 2014

Journal

The Plant Cell American Society of Plant Biologists

Book Volume: 26

Pages Range: 1838-1847

Journal Issue: 5

DOI: 10.1105/tpc.113.120071

Abstract

Peptide signals have emerged as an important class of regulators in cell-to-cell communication in plants. Several families of small, secreted proteins with a conserved C-terminal Pro-rich motif have been identified as functional peptide signals in Arabidopsis thaliana. These proteins are presumed to be trimmed proteolytically and undergo posttranslational modifications, such as hydroxylation of Pro residues and glycosylation, to form mature, bioactive signals. Identification and matching of such ligands with their respective receptors remains a major challenge since the genes encoding them often show redundancy and low expression restricted to a few cells or particular developmental stages. To overcome these difficulties, we propose the use of ectopic expression of receptor genes in suitable plant cells like Nicotiana benthamiana for testing ligand candidates in receptor output assays and in binding studies. As an example, we used the IDA peptide HAE/HSL2 receptor signaling system known to regulate floral organ abscission. We demonstrate that the oxidative burst response can be employed as readout for receptor activation by synthetic peptides and that a new, highly sensitive, nonradioactive labeling approach can be used to reveal a direct correlation between peptide activity and receptor affinity. We suggest that these approaches will be of broad value for the field of ligand-receptor studies in plants. © 2014 American Society of Plant Biologists. All rights reserved.

Authors with CRIS profile

Markus Albert

Additional Organisation(s)

Lehrstuhl für Molekulare Pflanzenphysiologie

Involved external institutions

Eberhard Karls Universität Tübingen DE Germany (DE) University of Oslo NO Norway (NO)

How to cite

APA:

Butenko, M.A., Wildhagen, M., Albert, M., Jehle, A., Kalbacher, H., Aalen, R.B., & Felix, G. (2014). Tools and strategies to match peptide-ligand receptor pairs. The Plant Cell, 26(5), 1838-1847. https://dx.doi.org/10.1105/tpc.113.120071

MLA:

Butenko, Melinka A., et al. "Tools and strategies to match peptide-ligand receptor pairs." The Plant Cell 26.5 (2014): 1838-1847.

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