Chemiluminescence-based detection of peptide activity and peptide-receptor binding in plants
Wildhagen M, Albert M, Butenko MA (2017)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2017
Journal
Publisher: Humana Press Inc.
Edited Volumes: Plant Genomics
Series: Methods in Molecular Biology
Book Volume: 1610
Pages Range: 287-295
DOI: 10.1007/978-1-4939-7003-2_18
Abstract
In living organisms, physical interaction of ligand molecules with their cognate receptors is an indispensable requirement for the initiation of cellular signaling pathways. To technically prove the biochemical interaction of ligands with their corresponding receptor, a biologically active but labeled peptide is required. Easily scorable bioassays, such as the production of reactive oxygen species, can be used to quantify the activity of a peptide. By using chemiluminescent probes, such as acridinium esters, as conjugates to label peptide ligands of interest, quantitative measurements of ligand-receptor binding can be performed in standard luminometers. Here we describe how this binding approach can be used to reveal peptide ligand-receptor binding in plant material.
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Germany (DE)
Norway (NO)How to cite
APA:
Wildhagen, M., Albert, M., & Butenko, M.A. (2017). Chemiluminescence-based detection of peptide activity and peptide-receptor binding in plants. In Wolfgang Busch (Eds.), Plant Genomics. (pp. 287-295). Humana Press Inc..
MLA:
Wildhagen, Mari, Markus Albert, and Melinka A. Butenko. "Chemiluminescence-based detection of peptide activity and peptide-receptor binding in plants." Plant Genomics. Ed. Wolfgang Busch, Humana Press Inc., 2017. 287-295.
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