Quantitative detection of oxidative burst upon activation of plant receptor kinases
Albert M, Fürst U (2017)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2017
Journal
Publisher: Humana Press Inc.
Edited Volumes: Plant Receptor Kinases
Series: Methods in Molecular Biology
Book Volume: 1621
Pages Range: 69-76
DOI: 10.1007/978-1-4939-7063-6_7
Abstract
The oxidative burst or the production of reactive oxygen species (ROS) is a typical cellular response of both plants and animals to diverse abiotic and biotic stresses. Mainly, the (re-)active oxygen species include the superoxide anion (O2−), hydrogen peroxide (H2O2), and the hydroxyl radical (OH•). Here, we outline the detection of extracellularly produced ROS in plant leaf pieces using a chemiluminescence-based bioassay with the luminol L-012 as a substrate being oxidized in the presence of ROS. Since this type of assay is in use in many laboratories, e.g., as a readout for activation of plant receptor kinases, we include a discussion on the interpretation of results and points addressing problems with the buffers at suboptimal pH values that negatively influence the chemiluminescence production.
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Germany (DE)How to cite
APA:
Albert, M., & Fürst, U. (2017). Quantitative detection of oxidative burst upon activation of plant receptor kinases. In Reidunn Birgitta Aalen (Eds.), Plant Receptor Kinases. (pp. 69-76). Humana Press Inc..
MLA:
Albert, Markus, and Ursula Fürst. "Quantitative detection of oxidative burst upon activation of plant receptor kinases." Plant Receptor Kinases. Ed. Reidunn Birgitta Aalen, Humana Press Inc., 2017. 69-76.
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