Podocyte density and albuminuria in aging diabetic ins2± mice with or without adenosine a1 receptor signaling

Faulhaber-Walter R, Jiang L, Mizel D, Zerfas PM, Kopp JB, Schnermann JB, Chen L, Schiffer M (2020)

Publication Type: Journal article

Publication year: 2020

Journal

International Journal of Nephrology and Renovascular Disease Dove Medical Press

Book Volume: 13

Pages Range: 19-26

DOI: 10.2147/IJNRD.S203810

Abstract

Aim of Study: To investigate podocyte density in aging diabetic Ins2± and Ins2±, A1AR-/-mouse models in C57Bl/6 background. Methods: Ins2± mice and especially Ins2±, adenosine A1 receptor knockout mice (Ins2±, A1AR-/-) are mouse models with a phenotype of diabetic nephropathy. Aged mice (at ~40 weeks) were assessed for glomerular filtration barrier function by measuring albuminuria, glomerular filtration, glomerular damage by electron microscopy, and podocyte numbers by Wilms Tumor protein (WT-1) staining. Results: Compared to healthy wild-type mice, both diabetic mouse models developed diabetic nephropathy, including hyperfiltration (p<0.01) and albuminuria (p<0.05). Typical diabetic structural glomerular and podocyte damage was visualized by electron microscopy. Podocyte count per glomerular area (podocyte density) was significantly decreased in both diabetic mouse models (p<0.01). In contrast, no significant correlation was detected between albuminuria and absolute podocyte count per glomerulus. Conclusion: The amount of albuminuria as marker of diabetic nephropathy does not correlate with the podocytes density; however, a relative podocyte deficiency became evident with an increase in glomerular area in the diabetic animals, suggesting a relative podocytopenia.

Authors with CRIS profile

Mario Schiffer Lehrstuhl für Innere Medizin IV

Involved external institutions

National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK / NIDDKD) US United States (USA) (US) Facharztzentrum Aarberg CH Switzerland (CH) Peking Union Medical College Hospital (PUMCH) / Beijing Xiehe Hospital CN China (CN)

How to cite

APA:

Faulhaber-Walter, R., Jiang, L., Mizel, D., Zerfas, P.M., Kopp, J.B., Schnermann, J.B.,... Schiffer, M. (2020). Podocyte density and albuminuria in aging diabetic ins2± mice with or without adenosine a1 receptor signaling. International Journal of Nephrology and Renovascular Disease , 13, 19-26. https://doi.org/10.2147/IJNRD.S203810

MLA:

Faulhaber-Walter, Robert, et al. "Podocyte density and albuminuria in aging diabetic ins2± mice with or without adenosine a1 receptor signaling." International Journal of Nephrology and Renovascular Disease 13 (2020): 19-26.

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