Tuning the structure of aminoferrocene-based anticancer prodrugs to prevent their aggregation in aqueous solution

Daum S, Babiy S, Konovalova H, Hofer W, Shtemenko A, Shtemenko N, Janko C, Alexiou C, Mokhir A (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Book Volume: 178

Pages Range: 9-17

DOI: 10.1016/j.jinorgbio.2017.08.038

Abstract

Aminoferrocene-based prodrugs are activated in cancer cells by reactive oxygen species (ROS). They were shown to exhibit high cytotoxicity towards a variety of cancer cell lines and primary cancer cells, but remain not toxic towards non-malignant cells. However, these prodrugs have rather high lipophilicity leading to relatively low water solubility. In particular, an n-octanol/water partition coefficient for the best aminoferrocene-based pro drug (2) was found to be 4.51 +/- 0.03. Though the approaches for decreasing lipophilicity are straightforward and include the addition of polar residues to the drug structure, these modifications also lead to dramatic decrease of cell permeability and, correspondingly, lower the activity of the drug. Therefore, a delicate balance of polar and unpolar groups should be found to reduce lipophilicity without compromising the useful drug properties. In this study we optimized an N-alkyl substituent, which is a key element responsible for the stabilization of the aminoferrocene drug released in cancer cells from prodrug 2. We found that an N-propargyl residue is an optimal replacement for the N-benzyl fragment. In particular, such a substitution (prodrug 7a) leads to reduction of prodrug lipophilicity down to logP = 3.78 +/- 0.05, improvement of its water solubility, decrease of its propensity towards aggregation and dramatic increase of its ROS-generating properties. Finally, we demonstrated that the optimized prodrug strongly suppresses growth of Guerin's carcinoma (T8) in vivo at the dose of 30 mg/kg.

Authors with CRIS profile

Involved external institutions

Ukrainian State University of Chemical Technology (USUCT) / Український державний хіміко-технологічний університет Ukraine (UA) National Academy of Sciences of Ukraine (NAN) / Національна академія наук України Ukraine (UA)

How to cite

APA:

Daum, S., Babiy, S., Konovalova, H., Hofer, W., Shtemenko, A., Shtemenko, N.,... Mokhir, A. (2018). Tuning the structure of aminoferrocene-based anticancer prodrugs to prevent their aggregation in aqueous solution. Journal of Inorganic Biochemistry, 178, 9-17. https://doi.org/10.1016/j.jinorgbio.2017.08.038

MLA:

Daum, Steffen, et al. "Tuning the structure of aminoferrocene-based anticancer prodrugs to prevent their aggregation in aqueous solution." Journal of Inorganic Biochemistry 178 (2018): 9-17.

BibTeX: Download