Phosphodiester hydrolysis computed for cluster models of enzymatic active sites
Batebi H, Imhof P (2016)
Publication Type: Journal article
Publication year: 2016
Journal
Book Volume: 135
Article Number: 262
Journal Issue: 12
DOI: 10.1007/s00214-016-2020-8
Abstract
Computation of phosphodiester hydrolysis in different models with one or two metal ions, representing typical active site architectures of nucleases, reveals an associative mechanism to be favorable in all of the cases studied in this work. Direct attack of the nucleophilic water molecule with proton transfer to the phosphate group is facilitated by an extra positive charge as provided by a metal ion located at the attack site or a positively charged histidine residue, whereas no such contribution can be observed on leaving group departure. A major catalytic effect is found by proton transfer from the nucleophilic water molecule to a histidine–aspartate cluster. Attack of the thus generated hydroxide ion on the phosphate group is just sufficiently stabilized by the metal ions to allow subsequent P–O bond dissociation.
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Germany (DE)How to cite
APA:
Batebi, H., & Imhof, P. (2016). Phosphodiester hydrolysis computed for cluster models of enzymatic active sites. Theoretical Chemistry Accounts, 135(12). https://doi.org/10.1007/s00214-016-2020-8
MLA:
Batebi, Hossein, and Petra Imhof. "Phosphodiester hydrolysis computed for cluster models of enzymatic active sites." Theoretical Chemistry Accounts 135.12 (2016).
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