FRET probes for measuring sphingolipid metabolizing enzyme activity

Mohamed ZH, Rhein C, Saied EM, Kornhuber J, Arenz C (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Chemistry and Physics of Lipids Elsevier

Book Volume: 216

Pages Range: 152-161

DOI: 10.1016/j.chemphyslip.2018.09.014

Abstract

Förster resonance energy transfer (FRET) probes are unique tools in biology, as they allow for a non-destructive monitoring of a certain state of a biomolecule or of an artificial substrate within living cells in real time. FRET substrates indicate their relative cleavage rate and thus the in situ activity of a given enzyme. In contrast to quenched probes or turn-on probes, one of the two separate signals of the FRET probes can be used as internal reference, which makes ratio-imaging and quantitation of cleavage events independent of cellular delivery possible. In this review, we describe the first examples of sphingolipid FRET probes in comparison to different alternative probes. Finally, we give an outlook on future probes and their potential application.

Authors with CRIS profile

Cosima Rhein Medizinische Fakultät Johannes Kornhuber Lehrstuhl für Psychiatrie und Psychotherapie

Involved external institutions

Humboldt-Universität zu Berlin DE Germany (DE)

How to cite

APA:

Mohamed, Z.H., Rhein, C., Saied, E.M., Kornhuber, J., & Arenz, C. (2018). FRET probes for measuring sphingolipid metabolizing enzyme activity. Chemistry and Physics of Lipids, 216, 152-161. https://doi.org/10.1016/j.chemphyslip.2018.09.014

MLA:

Mohamed, Zainelabdeen H., et al. "FRET probes for measuring sphingolipid metabolizing enzyme activity." Chemistry and Physics of Lipids 216 (2018): 152-161.

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