Anti-BAFF treatment interferes with humoral responses in a model of renal transplantation in rats
Steines L, Poth H, Schuster A, Geissler EK, Amann KU, Banas B, Bergler T (2019)
Publication Type: Journal article
Publication year: 2019
Journal
DOI: 10.1097/TP.0000000000002992
Abstract
BACKGROUND: B cell activating factor (BAFF) is associated with donor-specific antibodies (DSA) and poorer outcomes after renal transplantation (RTx). We examined the effects of anti-BAFF treatment on B cells, expression of costimulatory molecules and cytokines, germinal centers (GCs) and DSA formation in a RTx model in rats. METHODS: Anti-BAFF antibody was injected on days 3, 17, 31 and 45 after allogeneic RTx. Rats received reduced dose cyclosporine A (CsA) for 28 or 56 days to allow chronic rejection and DSA formation. Leukocytes, B cell subsets and DSA were measured using flow cytometry; expression of cytokines and costimulatory molecules was measured by qRT-PCR, and GCs and T follicular helper (TfH) were assessed using immunohistochemistry. Rejection was evaluated by a nephropathologist. RESULTS: Anti-BAFF treatment reduced the frequency of B cells in allografts and spleen. Naïve B cells were strongly reduced by anti-BAFF treatment in all compartments. mRNA expression of IL-6 and the costimulatory molecules CD40 and ICOS ligand was significantly reduced in anti-BAFF-treated rats. GCs were smaller and plasmablast/plasma cell numbers lower in anti-BAFF-treated rats, which was reflected by less DSA in certain IgG subclasses. CONCLUSIONS: Anti-BAFF treatment interferes with humoral responses at multiple levels in this model of allogeneic RTx.
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Germany (DE)How to cite
APA:
Steines, L., Poth, H., Schuster, A., Geissler, E.K., Amann, K.U., Banas, B., & Bergler, T. (2019). Anti-BAFF treatment interferes with humoral responses in a model of renal transplantation in rats. Transplantation. https://doi.org/10.1097/TP.0000000000002992
MLA:
Steines, Louisa, et al. "Anti-BAFF treatment interferes with humoral responses in a model of renal transplantation in rats." Transplantation (2019).
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