Role of guanylate binding protein-1 in vascular defects associated with chronic inflammatory diseases

Hammon M, Herrmann M, Bleiziffer O, Pryymachuk G, Andreoli L, Munoz LE, Amann KU, Mondini M, Gariglio M, Airo P, Schellerer V, Hatzopoulos AK, Horch RE, Kneser U, Stürzl M, Naschberger E (2011)

Publication Type: Journal article

Publication year: 2011

Journal

Journal of Cellular and Molecular Medicine Wiley-Blackwell / Wiley Open Access

Book Volume: 15

Pages Range: 1582-1592

Journal Issue: 7

DOI: 10.1111/j.1582-4934.2010.01146.x

Abstract

Rheumatic autoimmune disorders are characterized by a sustained pro-inflammatory microenvironment associated with impaired function of endothelial progenitor cells (EPC) and concomitant vascular defects. Guanylate binding protein-1 (GBP-1) is a marker and intracellular regulator of the inhibition of proliferation, migration and invasion of endothelial cells induced by several pro-inflammatory cytokines. In addition, GBP-1 is actively secreted by endothelial cells. In this study, significantly increased levels of GBP-1 were detected in the sera of patients with chronic inflammatory disorders. Accordingly we investigated the function of GBP-1 in EPC. Interestingly, stable expression of GBP-1 in T17b EPC induced premature differentiation of these cells, as indicated by a robust up-regulation of both Flk-1 and von Willebrand factor expression. In addition, GBP-1 inhibited the proliferation and migration of EPC in vitro. We confirmed that GBP-1 inhibited vessel-directed migration of EPC at the tissue level using the rat arterio-venous loop model as a novel quantitative in vivo migration assay. Overall, our findings indicate that GBP-1 contributes to vascular dysfunction in chronic inflammatory diseases by inhibiting EPC angiogenic activity via the induction of premature EPC differentiation. © 2011 The Authors Journal of Cellular and Molecular Medicine © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.

Authors with CRIS profile

Matthias Hammon Professur für Plastische Chirurgie und Handchirurgie Martin Herrmann Department of Medicine 3 – Rheumatology and Immunology Luis Munoz Becerra Department of Medicine 3 – Rheumatology and Immunology Kerstin Ute Amann Department of Nephropathology Vera Schellerer Department of Surgery Raymund Horch Professur für Plastische Chirurgie und Handchirurgie Michael Stürzl Professur für Molekulare und Experimentelle Chirurgie Elisabeth Naschberger Medizinische Fakultät

Involved external institutions

Università degli Studi di Brescia IT Italy (IT) Vanderbilt University US United States (USA) (US) University of Eastern Piedmont / Università degli Studi del Piemonte Orientale "Amedeo Avogadro" IT Italy (IT)

How to cite

APA:

Hammon, M., Herrmann, M., Bleiziffer, O., Pryymachuk, G., Andreoli, L., Munoz, L.E.,... Naschberger, E. (2011). Role of guanylate binding protein-1 in vascular defects associated with chronic inflammatory diseases. Journal of Cellular and Molecular Medicine, 15(7), 1582-1592. https://doi.org/10.1111/j.1582-4934.2010.01146.x

MLA:

Hammon, Matthias, et al. "Role of guanylate binding protein-1 in vascular defects associated with chronic inflammatory diseases." Journal of Cellular and Molecular Medicine 15.7 (2011): 1582-1592.

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