Konrad A, Jochmann R, Kühn E, Naschberger E, Chudasama P, Stürzl M (2011)
Publication Type: Book chapter / Article in edited volumes
Publication year: 2011
Publisher: Humana Press Inc.
Edited Volumes: Methods in Molecular Biology
Series: Methods in Molecular Biology
Book Volume: 706
Pages Range: 107-118
DOI: 10.1007/978-1-61737-970-3_9
Several human pathogenic viruses encode large genomes with often more than 100 genes. Viral pathogenicity is determined by carefully orchestrated co-operative activities of several different viral genes which trigger the phenotypic functions of the infected cells. Systematic analyses of these complex interactions require high-throughput transfection technology. Here we have provided a laboratory manual for the reverse transfected cell microarray (RTCM; alternative name: cell chip) as a high-throughput transfection procedure, which has been successfully applied for the systematic analyses of single and combination effects of genes encoded by the human herpesvirus-8 on the NF-kappaB signal transduction pathway. In order to quantitatively determine the effects of viral genes in transfected cells, protocols for the use of GFP as an indicator gene and for indirect immunofluorescence staining of cellular target proteins have been included. RTCM provides a useful methodological approach to investigate systematically combination effects of viral genes on cellular functions.
APA:
Konrad, A., Jochmann, R., Kühn, E., Naschberger, E., Chudasama, P., & Stürzl, M. (2011). Reverse transfected cell microarrays in infectious disease research. In Methods in Molecular Biology. (pp. 107-118). Humana Press Inc..
MLA:
Konrad, Andreas, et al. "Reverse transfected cell microarrays in infectious disease research." Methods in Molecular Biology. Humana Press Inc., 2011. 107-118.
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