Expression and localization of axin 2 in colorectal carcinoma and its clinical implication

Schaal U, Grenz S, Merkel S, Rau T, Hadjihannas M, Kremmer E, Chudasama P, Croner RS, Behrens J, Stürzl M, Naschberger E (2013)

Publication Type: Journal article

Publication year: 2013

Journal

International Journal of Colorectal Disease Springer Verlag

Book Volume: 28

Pages Range: 1469-1478

Journal Issue: 11

DOI: 10.1007/s00384-013-1709-6

Abstract

Purpose: Aberrant activation of the Wnt/β-catenin pathway plays a major role in the development of colorectal carcinoma (CRC). Axin 2 is a key protein of this pathway and is upregulated in CRC. Here, we investigated RNA- and protein expression of axin 2 in CRC tissues at the single cell level. Moreover, the association of axin 2 with prognosis and survival was investigated in a large cohort of CRC patients (n = 280). Methods: Localization and expression of axin 2 and β-catenin was investigated using in situ hybridization and immunohistochemical staining. The quantitative expression levels of axin 2 were determined using RT-qPCR. The association of axin 2 expression with prognosis and survival of the patients was determined by statistical analysis (logrank test, Kaplan-Meier). Results: Our results confirmed the upregulation of axin 2 in CRC and showed that it is broadly expressed in the cytoplasm of the tumor epithelial cells both, in the tumor center and at the invasion front. Axin 2 was rarely expressed by tumor stromal cells and only weakly by normal colonic epithelial cells. Staining of β-catenin and axin 2 in consecutive CRC tissue sections revealed that nuclear translocation of β-catenin in the tumor front was not associated with changes in the cytoplasmic localization of axin 2. Axin 2 did not show any association with proven prognostic factors or survival of the CRC patients. Conclusion: The generally increased expression of axin 2 in all tumor stages as compared to normal tissue suggests an initiating pathogenic function in the development of CRC. © 2013 Springer-Verlag Berlin Heidelberg.

Authors with CRIS profile

Ute Schaal Professur für Molekulare und Experimentelle Chirurgie Sandra Grenz Professur für Molekulare und Experimentelle Chirurgie Susanne Merkel Department of Surgery Michel Hadjihannas Lehrstuhl für Experimentelle Medizin II (Molekulare Tumorforschung) Priya Chudasama Professur für Molekulare und Experimentelle Chirurgie Jürgen Behrens Lehrstuhl für Experimentelle Medizin II (Molekulare Tumorforschung) Michael Stürzl Professur für Molekulare und Experimentelle Chirurgie Elisabeth Naschberger Medizinische Fakultät

Involved external institutions

Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (HMGU) / Helmholtz Munich DE Germany (DE)

How to cite

APA:

Schaal, U., Grenz, S., Merkel, S., Rau, T., Hadjihannas, M., Kremmer, E.,... Naschberger, E. (2013). Expression and localization of axin 2 in colorectal carcinoma and its clinical implication. International Journal of Colorectal Disease, 28(11), 1469-1478. https://doi.org/10.1007/s00384-013-1709-6

MLA:

Schaal, Ute, et al. "Expression and localization of axin 2 in colorectal carcinoma and its clinical implication." International Journal of Colorectal Disease 28.11 (2013): 1469-1478.

BibTeX: Download