Schaal U, Grenz S, Merkel S, Rau T, Hadjihannas M, Kremmer E, Chudasama P, Croner RS, Behrens J, Stürzl M, Naschberger E (2013)
Publication Type: Journal article
Publication year: 2013
Book Volume: 28
Pages Range: 1469-1478
Journal Issue: 11
DOI: 10.1007/s00384-013-1709-6
Purpose: Aberrant activation of the Wnt/β-catenin pathway plays a major role in the development of colorectal carcinoma (CRC). Axin 2 is a key protein of this pathway and is upregulated in CRC. Here, we investigated RNA- and protein expression of axin 2 in CRC tissues at the single cell level. Moreover, the association of axin 2 with prognosis and survival was investigated in a large cohort of CRC patients (n = 280). Methods: Localization and expression of axin 2 and β-catenin was investigated using in situ hybridization and immunohistochemical staining. The quantitative expression levels of axin 2 were determined using RT-qPCR. The association of axin 2 expression with prognosis and survival of the patients was determined by statistical analysis (logrank test, Kaplan-Meier). Results: Our results confirmed the upregulation of axin 2 in CRC and showed that it is broadly expressed in the cytoplasm of the tumor epithelial cells both, in the tumor center and at the invasion front. Axin 2 was rarely expressed by tumor stromal cells and only weakly by normal colonic epithelial cells. Staining of β-catenin and axin 2 in consecutive CRC tissue sections revealed that nuclear translocation of β-catenin in the tumor front was not associated with changes in the cytoplasmic localization of axin 2. Axin 2 did not show any association with proven prognostic factors or survival of the CRC patients. Conclusion: The generally increased expression of axin 2 in all tumor stages as compared to normal tissue suggests an initiating pathogenic function in the development of CRC. © 2013 Springer-Verlag Berlin Heidelberg.
APA:
Schaal, U., Grenz, S., Merkel, S., Rau, T., Hadjihannas, M., Kremmer, E.,... Naschberger, E. (2013). Expression and localization of axin 2 in colorectal carcinoma and its clinical implication. International Journal of Colorectal Disease, 28(11), 1469-1478. https://doi.org/10.1007/s00384-013-1709-6
MLA:
Schaal, Ute, et al. "Expression and localization of axin 2 in colorectal carcinoma and its clinical implication." International Journal of Colorectal Disease 28.11 (2013): 1469-1478.
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