Opportunities and challenges of single protein detection with iSCAT

Nicoli F, König K, Dahmardeh M, Gemeinhardt A, Mahmoodabadi RG, Mirzaalian Dastjerdi H, Köstler H, Sandoghdar V (2019)

Publication Type: Conference contribution

Publication year: 2019

Publisher: Institute of Electrical and Electronics Engineers Inc.

Conference Proceedings Title: 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019

Event location: Munich DE

ISBN: 9781728104690

DOI: 10.1109/CLEOE-EQEC.2019.8872414

Abstract

In recent decades large efforts have been made to push optical imaging to the limit, for example for detecting very small proteins. Great achievements have been obtained with fluorescence microscopy, both in terms of localization precision and study of the dynamics of different molecules. Despite the constant improvements, fluorescence-based methods suffer from intrinsic limitations, such as the finite number of emitted photons and photo damage, which limits the temporal resolution and the duration of a measurement. Furthermore, if the molecule of interest is intrinsically non-fluorescent, it has to be labelled with a suitable fluorophore, which in case of biological molecules could interfere with their activity and behavior.

Authors with CRIS profile

Katharina König Lehrstuhl für Experimentalphysik André Gemeinhardt Lehrstuhl für Experimentalphysik Houman Mirzaalian Dastjerdi Lehrstuhl für Informatik 10 (Systemsimulation) Harald Köstler Lehrstuhl für Informatik 10 (Systemsimulation) Vahid Sandoghdar Lehrstuhl für Experimentalphysik

Involved external institutions

Max-Planck-Institut für die Physik des Lichts (MPL) / Max Planck Institute for the Science of Light DE Germany (DE)

How to cite

APA:

Nicoli, F., König, K., Dahmardeh, M., Gemeinhardt, A., Mahmoodabadi, R.G., Mirzaalian Dastjerdi, H.,... Sandoghdar, V. (2019). Opportunities and challenges of single protein detection with iSCAT. In 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019. Munich, DE: Institute of Electrical and Electronics Engineers Inc..

MLA:

Nicoli, Francesca, et al. "Opportunities and challenges of single protein detection with iSCAT." Proceedings of the 2019 Conference on Lasers and Electro-Optics Europe and European Quantum Electronics Conference, CLEO/Europe-EQEC 2019, Munich Institute of Electrical and Electronics Engineers Inc., 2019.

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