Addition of decellularized extracellular matrix of porcine nasal cartilage improves cartilage regenerative capacities of PCL-based scaffolds in vitro

Wiggenhauser PS, Schwarz S, Körber L, Hoffmann TK, Rotter N (2019)

Publication Type: Journal article

Publication year: 2019

Journal

Journal of Materials Science: Materials in Medicine Springer

Book Volume: 30

Article Number: 121

Journal Issue: 11

DOI: 10.1007/s10856-019-6323-x

Abstract

Composite scaffolds can improve regenerative capacities of scaffolds in various tissue-engineering approaches. In order to generate a 3D printable scaffold that is capable of cartilage regeneration, decellularized extracellular matrix (DECM) of porcine nasal cartilage was added to 3D printed polycaprolactone (PCL) scaffolds. Subsequently, scaffolds (PCL, PCL/DECM and DECM) were seeded with human primary nasoseptal chondrocytes and differentiated with cartilage inductive medium for up to 42 days in vitro. Afterwards samples were analyzed with scanning electron microscopy, histology, biochemical assays and gene expression analysis. In short, results showed cell attachment and proliferation on all scaffolds. There was a trend towards ossification on pure PCL scaffolds, whereas we found evidence for cartilage tissue formation on DECM scaffolds as well as on PCL/DECM scaffolds. Moreover, biochemical analysis indicated an enhanced differentiation on novel PCL/DECM scaffolds. In conclusion, the addition of DECM to 3D printable PCL scaffolds may yield a new composite material for regenerative approaches in cartilage for facial reconstructive surgery. Further research will be necessary to evaluate these findings in vivo. [Figure not available: see fulltext.].

Authors with CRIS profile

Ludwig Körber Lehrstuhl für Bioverfahrenstechnik

Involved external institutions

Universitätsklinikum Ulm DE Germany (DE)

How to cite

APA:

Wiggenhauser, P.S., Schwarz, S., Körber, L., Hoffmann, T.K., & Rotter, N. (2019). Addition of decellularized extracellular matrix of porcine nasal cartilage improves cartilage regenerative capacities of PCL-based scaffolds in vitro. Journal of Materials Science: Materials in Medicine, 30(11). https://doi.org/10.1007/s10856-019-6323-x

MLA:

Wiggenhauser, P. S., et al. "Addition of decellularized extracellular matrix of porcine nasal cartilage improves cartilage regenerative capacities of PCL-based scaffolds in vitro." Journal of Materials Science: Materials in Medicine 30.11 (2019).

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