Cold atmospheric plasma causes a calcium influx in melanoma cells triggering CAP-induced senescence

Schneider C, Gebhardt L, Arndt S, Karrer S, Zimmermann JL, Fischer M, Boßerhoff AK (2018)

Publication Type: Journal article

Publication year: 2018

Journal

Scientific Reports Nature Publishing Group: Open Access Journals - Option B

Book Volume: 8

Journal Issue: 1

DOI: 10.1038/s41598-018-28443-5

Abstract

Cold atmospheric plasma (CAP) is a promising approach in anti-cancer therapy, eliminating cancer cells with high selectivity. However, the molecular mechanisms of CAP action are poorly understood. In this study, we investigated CAP effects on calcium homeostasis in melanoma cells. We observed increased cytoplasmic calcium after CAP treatment, which also occurred in the absence of extracellular calcium, indicating the majority of the calcium increase originates from intracellular stores. Application of previously CAP-exposed extracellular solutions also induced cytoplasmic calcium elevations. A substantial fraction of this effect remained when the application was delayed for one hour, indicating the chemical stability of the activating agent(s). Addition of ryanodine and cyclosporin A indicate the involvement of the endoplasmatic reticulum and the mitochondria. Inhibition of the cytoplasmic calcium elevation by the intracellular chelator BAPTA blocked CAP-induced senescence. This finding helps to understand the molecular influence and the mode of action of CAP on tumor cells.

Authors with CRIS profile

Christin Schneider Lehrstuhl für Biochemie und Molekulare Medizin Lisa Gebhardt Interdisziplinäres Zentrum für Klinische Forschung IZKF-Förderlinien Michael Fischer Medizinische Fakultät Anja Katrin Boßerhoff Lehrstuhl für Biochemie und Molekulare Medizin

Additional Organisation(s)

Emil-Fischer-Zentrum (Emil Fischer Center)

Involved external institutions

Universitätsklinikum Regensburg DE Germany (DE) terraplasma GmbH DE Germany (DE)

How to cite

APA:

Schneider, C., Gebhardt, L., Arndt, S., Karrer, S., Zimmermann, J.L., Fischer, M., & Boßerhoff, A.K. (2018). Cold atmospheric plasma causes a calcium influx in melanoma cells triggering CAP-induced senescence. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-28443-5

MLA:

Schneider, Christin, et al. "Cold atmospheric plasma causes a calcium influx in melanoma cells triggering CAP-induced senescence." Scientific Reports 8.1 (2018).

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