Ag modified mesoporous bioactive glass nanoparticles for enhanced antibacterial activity in 3D infected skin model

Zheng K, Balasubramanian P, Paterson TE, Stein R, MacNeil S, Fiorilli S, Vitale-Brovarone C, Shepherd J, Boccaccini AR (2019)

Publication Type: Journal article

Publication year: 2019

Journal

Book Volume: 103

Article Number: 109764

DOI: 10.1016/j.msec.2019.109764

Abstract

Bioactive glasses (BG)are versatile materials for various biomedical applications, including bone regeneration and wound healing, due to their bone bonding, antibacterial, osteogenic, and angiogenic properties. In this study, we aimed to enhance the antibacterial activity of SiO2-CaO mesoporous bioactive glass nanoparticles (MBGN)by incorporating silver (Ag)through a surface modification approach. The modified Ag-containing nanoparticles (Ag-MBGN)maintained spherical shape, mesoporous structure, high dispersity, and apatite-forming ability after the surface functionalization. The antibacterial activity of Ag-MBGN was assessed firstly using a planktonic bacteria model. Moreover, a 3D tissue-engineered infected skin model was used for the first time to evaluate the antibacterial activity of Ag-MBGN at the usage dose of 1 mg/mL. In the planktonic bacteria model, Ag-MBGN exhibited a significant antibacterial effect against both Pseudomonas aeruginosa and Staphylococcus aureus in comparison to non-engineered (Ag-free)MBGN and the blank control. Moreover, Ag-MBGN did not show cytotoxicity towards fibroblasts at the usage dose. However, in the 3D infected skin model, Ag-MBGN only demonstrated antibacterial activity against S. aureus whereas their antibacterial action against P. aeruginosa was inhibited. In conclusion, surface modification by Ag incorporation is a feasible approach to enhance the antibacterial activity of MBGN without significantly impacting their morphology, polydispersity, and apatite-forming ability. The prepared Ag-MBGN are attractive building blocks for the development of 3D antibacterial scaffolds for tissue engineering.

Authors with CRIS profile

Involved external institutions

University of Sheffield United Kingdom (GB) Politecnico di Torino Italy (IT)

How to cite

APA:

Zheng, K., Balasubramanian, P., Paterson, T.E., Stein, R., MacNeil, S., Fiorilli, S.,... Boccaccini, A.R. (2019). Ag modified mesoporous bioactive glass nanoparticles for enhanced antibacterial activity in 3D infected skin model. Materials Science and Engineering C, 103. https://doi.org/10.1016/j.msec.2019.109764

MLA:

Zheng, Kai, et al. "Ag modified mesoporous bioactive glass nanoparticles for enhanced antibacterial activity in 3D infected skin model." Materials Science and Engineering C 103 (2019).

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