Label-Free Multiphoton Endomicroscopy for Minimally Invasive In Vivo Imaging

Dilipkumar A, Al-Shemmary A, Kreiß L, Cvecek K, Carlé BE, Knieling F, Menezes JG, Thoma OM, Schmidt M, Neurath M, Waldner M, Friedrich O, Schürmann S (2019)

Publication Type: Journal article

Publication year: 2019


Book Volume: 6

Article Number: 1801735

Journal Issue: 8

DOI: 10.1002/advs.201801735


Multiphoton microscopy of cellular autofluorescence and second harmonic generation from collagen facilitates imaging of living cells and tissues without the need for additional fluorescent labels. Here, a compact multiphoton endomicroscope for label-free in vivo imaging in small animals via side-viewing needle objectives is presented. Minimal invasive imaging at cellular resolution is performed in colonoscopy of mice without surgical measures and without fluorescent dyes as a contrast agent. The colon mucosa is imaged repeatedly in the same animal in a mouse model of acute intestinal inflammation to study the process of inflammation at the tissue level within a time period of ten days, demonstrating the capabilities of label-free endomicroscopy for longitudinal studies for the first time.

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Dilipkumar, A., Al-Shemmary, A., Kreiß, L., Cvecek, K., Carlé, B.-E., Knieling, F.,... Schürmann, S. (2019). Label-Free Multiphoton Endomicroscopy for Minimally Invasive In Vivo Imaging. Advanced Science, 6(8).


Dilipkumar, Ashwathama, et al. "Label-Free Multiphoton Endomicroscopy for Minimally Invasive In Vivo Imaging." Advanced Science 6.8 (2019).

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