Chicken GRIFIN: binding partners, developmental course of localization and activation of its lens-specific gene expression by L-Maf/Pax6

Caballero GG, Schmidt S, Schnoelzer M, Schlötzer-Schrehardt U, Knospe C, Ludwig AK, Manning JC, Muschler P, Kaltner H, Kopitz J, Gabius HJ (2019)

Publication Type: Journal article

Publication year: 2019

Journal

Cell and Tissue Research Springer

Book Volume: 375

Pages Range: 665-683

Journal Issue: 3

DOI: 10.1007/s00441-018-2931-x

Abstract

Tissue lectins appear to be involved in a broad range of physiological processes, as reflected for the members of the family of galectins by referring to them as adhesion/growth-regulatory effectors. In order to clarify the significance of galectin presence, key challenges are to define their binding partners and the profile of localization. Having identified the chicken galectin-related interfiber protein (C-GRIFIN) as lens-specific protein present in the main body of adult lens, we here report its interaction with lens proteins in ligand blotting. The assumption for pairing with -, - and -crystallins was ascertained by mass spectrometric detection of their presence in eluted fractions obtained by affinity chromatography. Biochemical and immunohistochemical monitoring revealed protein presence from about 3-day-old embryos onwards, mostly in the cytoplasm of elongated posterior cells, later in secondary lens fiber cells. On the level of gene expression, its promoter was activated by transcription factor L-Maf alone and together with Pax6 like a crystallin gene, substantiating C-GRIFIN's status as lens-specific galectin. Using this combined strategy for counterreceptor and expression profiling by bio- and histochemical methods including light, electron and fluorescence microscopy, respective monitoring in lens development can now be taken to the level of the complete galectin family.

Authors with CRIS profile

Ursula Schlötzer-Schrehardt Medizinische Fakultät

Additional Organisation(s)

Department of Ophthalmology

Involved external institutions

Ludwig-Maximilians-Universität (LMU) DE Germany (DE) Ruprecht-Karls-Universität Heidelberg DE Germany (DE) Promega GmbH DE Germany (DE) Deutsches Krebsforschungszentrum (DKFZ) DE Germany (DE)

How to cite

APA:

Caballero, G.G., Schmidt, S., Schnoelzer, M., Schlötzer-Schrehardt, U., Knospe, C., Ludwig, A.-K.,... Gabius, H.-J. (2019). Chicken GRIFIN: binding partners, developmental course of localization and activation of its lens-specific gene expression by L-Maf/Pax6. Cell and Tissue Research, 375(3), 665-683. https://doi.org/10.1007/s00441-018-2931-x

MLA:

Caballero, Gabriel Garcia, et al. "Chicken GRIFIN: binding partners, developmental course of localization and activation of its lens-specific gene expression by L-Maf/Pax6." Cell and Tissue Research 375.3 (2019): 665-683.

BibTeX: Download