Gelatin coating increases in vivo bone formation capacity of three-dimensional 45S5 bioactive glass-based crystalline scaffolds

Westhauser F, Senger AS, Obert D, Ciraldo F, Schuhladen K, Schmidmaier G, Moghaddam A, Boccaccini AR (2019)


Publication Type: Journal article

Publication year: 2019

Journal

Book Volume: 13

Pages Range: 179-190

Journal Issue: 2

DOI: 10.1002/term.2780

Abstract

Recent studies have demonstrated that surface characteristics, porosity, and mechanical strength of three-dimensional 45S5-type bioactive glass (BG)-based scaffolds are directly correlated with osteogenic properties. Three-dimensional BG-based scaffolds obtained from maritime natural sponges (MNSs) as sacrificial templates exhibit the required morphological properties; however, no in vivo data about the osteogenic features are available. In this study, uncoated (Group A) and gelatin-coated (Group B) crystalline MNS-obtained BG-based scaffolds were evaluated mechanically and seeded with human mesenchymal stem cells prior to subcutaneous implantation in immunodeficient mice. Before implantation and after explantation, micro-computed tomography scans were conducted, and scaffolds were finally subjected to histomorphometry. Scaffolds of both groups showed bone formation. However, Group B scaffolds performed distinctly better as indicated by a significant increase in scaffold volume (8.95%, p = 0.039) over the implantation period compared with a nonsignificant increase of 5.26% in Group A scaffolds in micro-computed tomography analysis. Furthermore, percentage bone area was 10.33% (+/- 1.18%) in the Group B scaffolds, which was significantly (p = 0.007) higher compared with the 8.53% (+/- 0.77%) in the Group A scaffolds in histomorphometry. Compressive strength was enhanced significantly by gelatin coating (9 +/- 2 vs. 4 +/- 1 MPa; p = 0.029). The presence of gelatin on the remnant parts was verified by scanning electron microscopy and X-ray spectroscopy, demonstrating the coatings' resilience. MNS-obtained BG-based scaffolds were thus confirmed to exhibit osteogenic properties in vivo that can significantly be enhanced by gelatin coating.

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APA:

Westhauser, F., Senger, A.-S., Obert, D., Ciraldo, F., Schuhladen, K., Schmidmaier, G.,... Boccaccini, A.R. (2019). Gelatin coating increases in vivo bone formation capacity of three-dimensional 45S5 bioactive glass-based crystalline scaffolds. Journal of Tissue Engineering and Regenerative Medicine, 13(2), 179-190. https://doi.org/10.1002/term.2780

MLA:

Westhauser, Fabian, et al. "Gelatin coating increases in vivo bone formation capacity of three-dimensional 45S5 bioactive glass-based crystalline scaffolds." Journal of Tissue Engineering and Regenerative Medicine 13.2 (2019): 179-190.

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