Signal Amplification Strategy Based on TiO 2 -Nanotube Layers and Nanobeads Carrying Quantum Dots for Electrochemiluminescent Immunosensors

Gao ZD, Zhuang QL, Song YY, Lee K, Schmuki P (2013)

Publication Type: Journal article

Publication year: 2013

Journal

ChemistryOpen Wiley Open Access: Chemistry Open / Wiley

Book Volume: 2

Pages Range: 93-98

Journal Issue: 3

DOI: 10.1002/open.201300003

Abstract

Self-organized TiO2-nanotube layers can be used for immunoassay-type sensing in combination with amplifying CdTe labels in a direct and very sensitive electrochemiluminescent (ECL) configuration. Key properties for this method are the conductivity of the TiO2-nanotubes, and their transparency for light emitted from the CdTe labels at approximately 2.4 eV. To demonstrate the potential of this platform, we constructed a sandwich-type immunoassay onto the TiO2-nanotube wall with a layer of (3-aminopropyl)triethoxysilane as the cross-linker for antibody immobilization. For the counter part of the sandwich, we created an amplification system consisting of TiO2 nano-beads carrying the secondary antibody and multiple CdTe quantum dots (multiQD). For antigen (IgG) detection, we find that this combination of 3D transparent electrode with multi-QD labels allows for an ECL detection limit of 0.05 pgmL1 a linearity of the signal in the range of 0.1--108 pg mL-1

Authors with CRIS profile

Kiyoung Lee Lehrstuhl für Werkstoffwissenschaften (Korrosion und Oberflächentechnik) (LKO) Patrik Schmuki Lehrstuhl für Werkstoffwissenschaften (Korrosion und Oberflächentechnik) (LKO)

Additional Organisation(s)

Exzellenz-Cluster Engineering of Advanced Materials (EAM)

Involved external institutions

Northeastern University US United States (USA) (US)

How to cite

APA:

Gao, Z.-D., Zhuang, Q.-L., Song, Y.-Y., Lee, K., & Schmuki, P. (2013). Signal Amplification Strategy Based on TiO 2 -Nanotube Layers and Nanobeads Carrying Quantum Dots for Electrochemiluminescent Immunosensors. ChemistryOpen, 2(3), 93-98. https://doi.org/10.1002/open.201300003

MLA:

Gao, Zhi-Da, et al. "Signal Amplification Strategy Based on TiO 2 -Nanotube Layers and Nanobeads Carrying Quantum Dots for Electrochemiluminescent Immunosensors." ChemistryOpen 2.3 (2013): 93-98.

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